Micro RNA-423 rs6505162 C>A polymorphism in colorectal cancer: Relation to susceptibility and metastasis

Abstract

Background

Colorectal cancer (CRC) is the second most deadly cancer. There is an urgent need to identify biomarkers for early diagnosis and prognosis of the disease. Micro-RNAs (miRNAs) are molecules that regulate gene expression at the post-transcriptional level. The last decade had witnessed tremendous progress in the field of miRNAs and cancer including CRC. The purpose of this research was to investigate the potential association of the micro-RNA −423 rs6505162 gene variant with susceptibility to colorectal cancer in a cohort of Egyptian patients.

Methods

The real-time polymerase chain reaction (real-time PCR) method was used to genotype 120 colorectal carcinoma patients and 120 matched healthy controls.

Results

Compared with CC homozygote, the AA homozygote and the combination of AC and AA genotype exhibited a significantly decreased risk of colorectal carcinoma (odds ratio = 0.298, p-value = 0.033, 95 %CI (0.098–0.910); odds ratio = 0.558, p-value = 0.027, 95 %CI (0.333–0.936), correspondingly). Furthermore, CC genotype showed a significant association with the frequency of lymph node infiltration and metastasis in patients compared to the AC + AA group.

Conclusion

Our study suggested that miR-423 rs6505162 C > A polymorphism may serve as a protective factor in the development of colorectal cancer in Egyptian patients and miR-423 rs6505162 C > A genotype showed significant association with metastasis in CRC patients.