Imaging Ultraweak Photon Emission from Living and Dead Mice and from Plants under Stress

IF 4.8 2区化学 Q2 CHEMISTRY, PHYSICAL

The Journal of Physical Chemistry Letters Pub Date : 2025-04-24 DOI:10.1021/acs.jpclett.4c03546

V. Salari, V. Seshan, L. Frankle, D. England, C. Simon, D. Oblak

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Abstract

The phenomenon of biological ultraweak photon emission (UPE), that is, extremely low-intensity emission (10–10³ photons cm^–2 s^–1) in the spectral range of 200–1000 nm, has been observed in all living systems that have been examined. Here, we report experiments that exemplify the ability of novel imaging systems to detect variations in UPE for a set of physiologically important scenarios. We use electron-multiplying charge-coupled device (EMCCD) and charge-coupled device (CCD) cameras to capture single visible-wavelength photons with low noise and quantum efficiencies higher than 90%. Our investigation reveals significant contrast between the UPE from live vs dead mice. In plants, we observed that an increase in the temperature and injuries both caused an increase in UPE intensity. Moreover, chemical treatments modified the UPE emission characteristics of plants, particularly the application of a local anesthetic (benzocaine) to injury, which showed the highest emission among the compounds tested. As a result, UPE imaging provides the possibility of non-invasive label-free imaging of vitality in animals and the responses of plants to stress.

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胁迫下活鼠、死鼠及植物的超弱光子发射成像

生物超弱光子发射（UPE）现象，即在200-1000 nm光谱范围内的极低强度发射（10-103光子cm-2 s-1），已经在所有被研究的生命系统中观察到。在这里，我们报告了一些实验，这些实验证明了新型成像系统在一系列生理上重要的场景中检测UPE变化的能力。我们利用电子倍增电荷耦合器件（EMCCD）和电荷耦合器件（CCD）相机捕获了低噪声、量子效率高于90%的单可见波长光子。我们的研究揭示了活鼠和死鼠的UPE之间的显著差异。在植物中，我们观察到温度和伤害的增加都会导致UPE强度的增加。此外，化学处理改变了植物的UPE排放特征，特别是局部麻醉剂（苯佐卡因）对损伤的应用，在测试的化合物中显示出最高的排放。因此，UPE成像为动物活力和植物对压力的反应提供了无创无标签成像的可能性。

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来源期刊

The Journal of Physical Chemistry Letters CHEMISTRY, PHYSICAL-NANOSCIENCE & NANOTECHNOLOGY

CiteScore

9.60

自引率

7.00%

发文量

1519

审稿时长

1.6 months

期刊介绍： The Journal of Physical Chemistry (JPC) Letters is devoted to reporting new and original experimental and theoretical basic research of interest to physical chemists, biophysical chemists, chemical physicists, physicists, material scientists, and engineers. An important criterion for acceptance is that the paper reports a significant scientific advance and/or physical insight such that rapid publication is essential. Two issues of JPC Letters are published each month.