CeO₂ and Mn₃O₄-based nanozymes exhibit scavenging of singlet oxygen species and hydroxyl radicals

Abstract

Singlet oxygen and hydroxyl radicals are highly reactive species that contribute significantly to the oxidative stress-related pathologies. Herein, we report the effective scavenging of 1O2 and •OH by CeO₂ and Mn₃O₄-based nanozymes and compare with the well-known scavengers of these radicals. The IC50 values of scavenging of •OH by nanozymes was compared with NAC, which was in the order of Mn3O4 [2.3 µM] < CeO2 [5.4 µM] < NAC [28.4 µM]. Similarly, the IC50 values for 1O2 scavenging was observed as Mn3O4 [4.75 µM] < Sodium Azide [60.5 µM] < CeO2 [857.27 µM]. The cell viability assays, ROS generation studies and cell cycle analysis revealed that these nanozymes (1µg/mL) are biocompatible to the mammalian cells.