Microbeam irradiation reveals cytoplasmic damage contributes to micronuclei induction

IF 2.8 3区物理与天体物理 Q2 PHYSICS, MULTIDISCIPLINARY

The European Physical Journal Plus Pub Date : 2025-04-23 DOI:10.1140/epjp/s13360-025-06265-5

Teruaki Konishi, Jun Wang, Alisa Kobayashi, Cuihua Liu, Yota Hiroyama, Taisei Mamiya, Tamon Kusumoto, Masakazu Oikawa

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Abstract

Microbeam technology offers a valuable tool for investigating inter- and intracellular responses to radiation-induced damage. This study investigated the contribution of cytoplasmic irradiation to micronuclei (MN) induction using proton microbeams. Three irradiation conditions were employed: (N) nucleus-only, (C) cytoplasm-only, and (N + C) irradiating both. In N + C, 1000 protons were delivered to the cytoplasm. For both N and N + C, MN induction increased proportionally with nuclear proton dose (Np) within the 0 ≤ Np ≤ 100 range. However, the N + C slope was 2/3 of the N slope. N cells exhibited peak MN induction at 150 protons, while N + C peaked at 300 protons. Cytoplasmic irradiation alone also induced MNs, but with a lower slope and reaching a plateau after Np = 300. These results demonstrate that cytoplasmic damage not only contributes to MN induction but also appears to promote protective cellular responses. This highlights the complex interplay between cellular organelles during radiation exposure and emphasizes the importance of considering cytoplasmic contributions to cellular radioresistance.

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微束辐照显示细胞质损伤有助于微核诱导

微束技术为研究细胞间和细胞内对辐射损伤的反应提供了有价值的工具。本文研究了质子微束辐照对微核诱导的作用。采用三种辐照条件：(N)只辐照细胞核，(C)只辐照细胞质，（N + C）同时辐照两者。在N + C中，1000个质子被传递到细胞质中。在0≤Np≤100范围内，N和N + C对MN的诱导均随核质子剂量（Np）成比例增加。而N + C的斜率是N斜率的2/3。N细胞在150个质子时MN诱导达到峰值，而N + C细胞在300个质子时MN诱导达到峰值。单独的细胞质辐照也能诱导MNs，但斜率较低，在Np = 300后达到平稳期。这些结果表明，细胞质损伤不仅有助于MN诱导，而且似乎促进保护性细胞反应。这突出了辐射暴露期间细胞器之间复杂的相互作用，并强调了考虑细胞质对细胞辐射抗性的贡献的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

The European Physical Journal Plus PHYSICS, MULTIDISCIPLINARY-

CiteScore

5.40

自引率

8.80%

发文量

1150

审稿时长

4-8 weeks

期刊介绍： The aims of this peer-reviewed online journal are to distribute and archive all relevant material required to document, assess, validate and reconstruct in detail the body of knowledge in the physical and related sciences. The scope of EPJ Plus encompasses a broad landscape of fields and disciplines in the physical and related sciences - such as covered by the topical EPJ journals and with the explicit addition of geophysics, astrophysics, general relativity and cosmology, mathematical and quantum physics, classical and fluid mechanics, accelerator and medical physics, as well as physics techniques applied to any other topics, including energy, environment and cultural heritage.