Transcriptome sequence reveal the roles of MaGME777 and MabHLH770 in drought tolerance in Musa acuminata

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-04-19 DOI:10.1016/j.plantsci.2025.112495

Yuchen Wei , Jingfang Shi , Xueyi Xie , Feng Zhang , Huizhen Dong , Yaoyao Li , Fangcheng Bi , Xiaosan Huang , Tongxin Dou

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引用次数: 0

Abstract

Banana, a globally cultivated fruit, faces significant constraints in distribution and sustainable production due to drought stress. This study investigated drought tolerance in Cavendish bananas using RNA-seq time-course analysis and molecular biology experiments. Plants were subjected to dehydration treatments, and physiological indicators such as electrolyte leakage, proline content, malonaldehyde, peroxidase activity, and hydrogen peroxide content were assessed. RNA-Seq and qRT-PCR were used to analyze transcriptional changes under drought. Weighted gene co-expression network (WGCNA) analysis identified thousands of differentially expressed genes (DEGs) at different time points, with a core set of 2660 DEGs consistently identified. KEGG enrichment analysis revealed MaGME777, a glycolysis/gluconeogenesis gene, as a potential drought resistance regulator. Virus-mediated gene silencing (VIGS) of MaGME777 reduced drought tolerance in bananas. Yeast one-hybrid (Y1H) and luciferase reporter assays demonstrated that the transcription factor MabHLH770 directly binds and activates the MaGME777 promoter. VIGS downregulation of MabHLH770 also reduced drought tolerance. In conclusion, this study revealed that MabHLH770 is a positive regulator of drought stress, by targeting MaGME777 promoter and activating their expression to enhance drought tolerance. These findings provide a foundation for developing drought-resistant banana cultivars through molecular breeding approaches.

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转录组测序揭示了MaGME777和MabHLH770基因在针叶芭蕉抗旱性中的作用

香蕉是一种全球种植的水果，由于干旱胁迫，香蕉在分布和可持续生产方面面临重大限制。利用RNA-seq时程分析和分子生物学实验研究了卡文迪什香蕉的抗旱性。对植株进行脱水处理，评估其电解质泄漏、脯氨酸含量、丙二醛、过氧化物酶活性和过氧化氢含量等生理指标。利用RNA-Seq和qRT-PCR分析干旱条件下的转录变化。加权基因共表达网络（WGCNA）分析在不同时间点鉴定出数千个差异表达基因（deg），一致鉴定出2660个核心deg。KEGG富集分析显示，糖酵解/糖异生基因MaGME777是潜在的抗旱调节因子。MaGME777病毒介导的基因沉默（VIGS）降低了香蕉的抗旱性。酵母单杂交（Y1H）和荧光素酶报告基因实验表明，转录因子MabHLH770直接结合并激活MaGME777启动子。MabHLH770的VIGS下调也降低了耐旱性。综上所述，本研究表明MabHLH770是干旱胁迫的正向调节因子，通过靶向MaGME777启动子并激活其表达来增强抗旱性。这些发现为通过分子育种方法培育香蕉抗旱品种提供了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.