Enhancing lutein concentration in an indigenous microalgal strain through salinity, light intensity and nutrient concentrations and evaluation of its anticancer potential

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2025-04-17 DOI:10.1016/j.algal.2025.104054

Arima Marchese , Serena Lima , Valeria Villanova , Eleonora Montuori , Daniele De Luca , Chiara Lauritano , Francesca Scargiali

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Abstract

The search for new approaches for treating cancer is an urgent need and microalgae have recently shown to be an underestimated source of biocompounds. In this work, the anticancer potential of an indigenous Chlorella-like microalgal strain was investigated by optimizing its lutein content using a Design of Experiment (DoE) approach. Several parameters were assessed such as NaCl, phosphate and nitrate concentration in combination with light intensity. Sodium chloride and nitrate concentrations showed antagonist effects on lutein accumulation. The crude methanolic extracts of the obtained microalgal cultures, containing 2.15 ± 0.12, 1.70 ± 0.17 and of 0.68 ± 0.09 μg mg⁻¹ of lutein, were tested on cancer cell lines, revealing a dosage-dependent antiproliferative effect on melanoma A2058 cells. The extracts' fractions containing lutein exhibited similar effects, likely due to a concerted action of multiple substances, with lutein being one of the main contributors. The described approach, which combined bioprocess optimization and assessment of anticancer proprieties, showed a significant potential in the discovery of new bioactives for pharmaceutics.

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通过盐度、光照强度和营养浓度提高本土微藻叶黄素浓度及其抗癌潜力评估

寻找治疗癌症的新方法是迫切需要的，微藻最近被证明是一种被低估的生物化合物来源。本研究采用实验设计（Design of Experiment, DoE）的方法，对一株本土小球藻样微藻菌株的叶黄素含量进行优化，研究其抗癌潜力。考察了NaCl、磷酸盐和硝酸盐浓度与光照强度的关系。氯化钠和硝酸盐浓度对叶黄素积累具有拮抗作用。微藻培养物粗甲醇提取物叶黄素含量分别为2.15±0.12、1.70±0.17和0.68±0.09 μg mg−1，对肿瘤细胞系进行了实验，结果显示其对黑色素瘤A2058细胞具有剂量依赖性的抗增殖作用。含有叶黄素的提取物部分也表现出类似的效果，可能是多种物质协同作用的结果，叶黄素是主要的贡献者之一。该方法结合了生物工艺优化和抗癌特性的评估，在发现新的药物生物活性方面显示出巨大的潜力。

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来源期刊

Algal Research-Biomass Biofuels and Bioproducts BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

9.40

自引率

7.80%

发文量

332

期刊介绍： Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment