Galectin-1-targeted theranostic nanoprobe for multimodal imaging and photothermal therapy in cervical cancer lymph node metastases

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-04-21 DOI:10.1016/j.colsurfb.2025.114714

Hui Duan , Huimin Li , Qianjun Su , Ping Liu , Mengdan Zhang , Xiaolin Chen , Ruiyuan Liu , Pengfei Li , Chunlin Chen

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引用次数: 0

Abstract

Accurately evaluating lymph node (LN) status is essential for cervical cancer staging and making treatment decisions. However, the current preoperative imaging modalities for diagnosing LN metastasis in patients with cervical cancer are suboptimal, and the existing radiochemotherapy methods lack specificity, which leads to increased toxicity and side effects. To overcome these limitations, we aimed to develop a theranostic strategy for LN metastases with a galectin-1-targeted nanoprobe for dual-modal magnetic resonance imaging (MRI)/near-infrared (NIR) imaging and photothermal therapy (PTT). The PEG@MnPb-IR820 nanoparticles were synthesized through an integrated approach combining microemulsion, thin-film hydration, and esterification coupling methods. The nanoparticles were synthesized and characterized for their physicochemical properties and biosafety. After extensive in vitro and in vivo experiments, the TDG@PMI NPs demonstrated low biological toxicity, excellent stability, and a diameter of 74 nm, which is suitable for LN accumulation. With effective MRI and NIR imaging guidance, this nanoprobe displayed outstanding anticancer activity in tumors and metastatic sentinel LNs upon single NIR laser irradiation by inducing mitochondrial apoptosis in tumor cells.

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半凝集素-1靶向治疗纳米探针用于宫颈癌淋巴结转移的多模态成像和光热治疗

准确评估淋巴结（LN）状态对宫颈癌分期和治疗决策至关重要。然而，目前诊断宫颈癌患者淋巴结转移的术前影像学方法并不理想，现有放化疗方法缺乏特异性，导致毒副作用增加。为了克服这些局限性，我们的目标是开发一种针对半乳糖凝集素-1的纳米探针用于双模态磁共振成像(MRI)/近红外（NIR）成像和光热治疗（PTT）的LN转移治疗策略。采用微乳液、薄膜水化和酯化耦合相结合的方法合成了PEG@MnPb-IR820纳米颗粒。合成了纳米颗粒，并对其理化性质和生物安全性进行了表征。经过大量的体外和体内实验，TDG@PMI NPs的生物毒性低，稳定性好，直径为74 nm，适合LN积累。在有效的MRI和近红外成像指导下，该纳米探针通过诱导肿瘤细胞线粒体凋亡，在单次近红外激光照射下对肿瘤和转移前哨淋巴结显示出出色的抗癌活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.