High temperature-responsive DEAR4 condensation confers thermotolerance through recruiting TOPLESS in Arabidopsis nucleus

IF 6.2 1区生物学 Q1 PLANT SCIENCES

The Plant Journal Pub Date : 2025-04-23 DOI:10.1111/tpj.70172

Qi Wang, Zhen Gong, Ziqiang Zhu

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引用次数: 0

Abstract

Global warming is harmful to plants and threatens crop yields in the world. In contrast to other abiotic stresses, the molecular mechanisms for plant high temperature perception and signaling are still not fully understood. Here, we report that transcription factor DREB AND EAR MOTIF PROTEIN 4 (DEAR4) positively regulates heat tolerance in Arabidopsis thaliana. We further reveal that DEAR4 proteins undergo liquid–liquid phase separation (LLPS) and high temperature could induce DEAR4 condensate formation in the nucleus. Moreover, DEAR4 recruits the transcriptional co-repressor TOPLESS (TPL) into the nuclear speckles under high temperature. The high temperature triggered DEAR4-TPL co-condensates enhance their transcriptional repression activity through modulating histone deacetylation levels of GASA5, which is a reported negative regulator of HEAT SHOCK PROTEINs (HSPs). A genome-wide transcriptional landscape study confirms that DEAR4 induces the expression of multiple HSPs. Taken together, we illustrate a transcriptional repression mechanism mediated by DEAR4 through LLPS to confer plants thermotolerance and open a new avenue for translating this knowledge into crops for improving their heat resistance.

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高温响应型DEAR4缩合通过在拟南芥细胞核中募集toppless而获得耐热性

全球变暖对植物有害，并威胁着全世界的作物产量。与其他非生物胁迫相比，植物对高温的感知和信号传导的分子机制仍不完全清楚。在这里，我们报告了转录因子 DREB AND EAR MOTIF PROTEIN 4（DEAR4）对拟南芥耐热性的正向调节作用。我们进一步发现，DEAR4 蛋白会发生液-液相分离（LLPS），高温会诱导细胞核中 DEAR4 凝聚物的形成。此外，在高温条件下，DEAR4会将转录共抑制因子TOPLESS（TPL）招募到核斑点中。高温触发的 DEAR4-TPL 共凝集物通过调节 GASA5 的组蛋白去乙酰化水平来增强其转录抑制活性，而 GASA5 据报道是热休克蛋白（HSP）的负调控因子。一项全基因组转录景观研究证实，DEAR4 可诱导多种 HSPs 的表达。综上所述，我们说明了 DEAR4 通过 LLPS 介导的转录抑制机制赋予植物耐热性，并为将这一知识转化为作物以提高其耐热性开辟了一条新途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

The Plant Journal 生物-植物科学

CiteScore

13.10

自引率

4.20%

发文量

415

审稿时长

2.3 months

期刊介绍： Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.