Development of an ultrasensitive magnetic bead-based non-competitive chemiluminescent immunoassay for 17β-estradiol in milk

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-04-22 DOI:10.1007/s00604-025-07175-0

Yi-Xian Liu, Bao-Zhu Jia, Xu-Chao Zhang, Fei Hou, Jun-Peng Liu, Wei-Yun Guo, Yuan Meng, Zhen-Lin Xu, Lin Luo

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引用次数: 0

Abstract

Chronic dietary intake of 17β-estradiol (E₂) poses significant health risks, necessitating rapid and accurate analytical techniques for effective surveillance. This study presents a magnetic particle (MP)-assisted non-competitive chemiluminescent immunoassay (NCLIA) designed for E₂ quantification in milk. The NCLIA employs anti-E₂ monoclonal antibody (mAb)-coated MPs to capture E₂ target molecules, followed by the recognition of acridinium ester (AE)-conjugated anti-E₂@mAb immunocomplex antibody. Magnetic separation facilitates efficient isolation of immunocomplexes before automated luminescence measurement. This NCLIA demonstrated superior performance, without the need for complex pretreatment of milk samples, significantly simplifying the analysis process. The detection limit was as low as 1.57 pg/mL, and total detection time was 8 min. Additionally, NCLIA’s results were highly consistent with those obtained by liquid chromatography-tandem mass spectrometry (LC–MS/MS), validating their reliability in analyzing real samples. Therefore, this study presents an efficient and reliable tool for the rapid screening and detection of 17β-estradiol in milk, which holds significant value for food safety monitoring.

Graphical abstract

查看原文本刊更多论文

牛奶中17β-雌二醇超灵敏磁珠非竞争化学发光免疫分析方法的建立

长期膳食摄入17β-雌二醇（E2）会带来重大健康风险，需要快速准确的分析技术进行有效监测。本研究提出了一种磁粒辅助非竞争化学发光免疫分析法（NCLIA），用于牛奶中E2的定量分析。NCLIA采用抗E2单克隆抗体（mAb）包被MPs捕获E2靶分子，然后识别吖啶酸酯（AE）偶联anti-E2@mAb免疫复合物抗体。磁分离有助于在自动发光测量之前有效地分离免疫复合物。该NCLIA表现出优异的性能，无需对牛奶样品进行复杂的预处理，大大简化了分析过程。检出限低至1.57 pg/mL，总检测时间为8 min。此外，NCLIA的结果与液相色谱-串联质谱（LC-MS /MS）的结果高度一致，验证了其在实际样品分析中的可靠性。因此，本研究为牛奶中17β-雌二醇的快速筛选和检测提供了一种高效可靠的工具，对食品安全监测具有重要价值。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.