Interface self-assembly preparation of Nylon66-Ag for monitoring of H2O2 and AA in serum

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-04-14 DOI:10.1016/j.saa.2025.126238

Ziting Ye , Yujun Zheng , Shuyan Ruan , Jiajia Liu , Ying Li , Chuyi Wang , Yudong Lu , Ruiyun You , Liren Xiao , Jingxin Chen , Yaling Wu , Jingbo Chen , Lanjin Zhu , Zixing Yan

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引用次数: 0

Abstract

Monitoring the redox status in vivo is important for maintaining redox homeostasis and understanding related pathological processes. In this study, based on an interfacial self-assembly method, a silver-coated nylon film (Nylon66-Ag) was prepared as a SERS substrate and a single-molecule specific SERS probe, 2-mercapto-1,4-diphenol (2-MHQ), was modified on the silver nanoparticles for sensitive detection of H₂O₂ and AA in human serum. Remarkably, this method, without the introduction of internal standard signaling molecules, only one Raman signaling reporter molecule, 2-MHQ, was able to sensitively detect H₂O₂ and AA, and thus monitor the redox reactions in human serum, with LODs as low as 4.03 × 10⁻¹⁰ M for H₂O₂ and 9.42 × 10⁻⁹ M for AA. The detection limits are significantly lower than the concentration ranges of endogenous H₂O₂ and AA in the blood of healthy individuals. The SERS strategy proposed in this paper is expected to be used to study signaling pathways related to redox kinetics.

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用于监测血清中H2O2和AA的尼龙66- ag界面自组装制备

监测体内氧化还原状态对于维持氧化还原稳态和了解相关病理过程非常重要。本研究基于界面自组装的方法，制备了镀银尼龙薄膜（Nylon66-Ag）作为SERS底物，并将其修饰为单分子特异性SERS探针2-巯基-1,4-二酚（2-MHQ），用于人血清中H2O2和AA的灵敏检测。值得注意的是，该方法在不引入内部标准信号分子的情况下，只有一个拉曼信号报告分子2-MHQ能够灵敏地检测H2O2和AA，从而监测人血清中的氧化还原反应，H2O2的lod低至4.03 × 10−10 M， AA的lod低至9.42 × 10−9 M。检测限明显低于健康人血液中内源性H2O2和AA浓度范围。本文提出的SERS策略有望用于研究与氧化还原动力学相关的信号通路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.