Identification of PP2C gene family in moso bamboo (Phyllostachys edulis) and function analysis of PhePP2CA13

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-04-17 DOI:10.1016/j.plaphy.2025.109929

Qihan Lin, Xin Shan, Qin Si, Yidong Liu, Min Wu

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Abstract

The protein phosphatase 2C (PP2C) gene family plays an important role in plant growth and development and resistance to abiotic stresses. In our research, the PP2C family containing 103 genes was comprised eleven distinct subfamilies in moso bamboo (Phyllostachys edulis). PP2C gene family mainly expand through segmental duplications and basically underwent purifying selection in moso bamboo. PP2CA subfamily, the most extensively studied subfamily, carried 19 members in moso bamboo PP2CA subfamily. The cis-acting elements analysis and expression patterns of PhePP2CA genes indicated their potential functions in response to abiotic stress. Next, we gained the lines of transgenic PhePP2CA13-overexpressing plants. Overexpression of PhePP2CA13 significantly decreased tolerance to drought stress, along with a lower germination rate and suppressed root length under drought treatment. Moreover, OE lines also reduced the sensitivity to exogenous ABA in OE lines. More importantly, yeast one-hybrid assays demonstrated that PhePP2CA13 acted downstream of PhebZIP47, and Dual-luciferase assays further showed that the expression of PhePP2CA13 was inhibited by PhebZIP47. This study enriched the knowledge of PP2CA genes and provided genetic resources for stress resistant breeding in moso bamboo.

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毛竹PP2C基因家族的鉴定及pppp2ca13的功能分析

蛋白磷酸酶2C （PP2C）基因家族在植物的生长发育和对非生物胁迫的抗性中起着重要作用。在我们的研究中，毛竹（Phyllostachys edulis）中含有103个基因的PP2C家族被划分为11个不同的亚科。在毛竹中，PP2C基因家族主要通过片段复制进行扩增，并基本经过纯化选择。PP2CA亚家族是研究最广泛的一个亚家族，在毛竹PP2CA亚家族中有19个成员。通过对PhePP2CA基因的顺式作用元件分析和表达模式分析，揭示了其在应对非生物胁迫中的潜在功能。接下来，我们获得了过表达phepp2ca13的转基因植株品系。过表达的PhePP2CA13显著降低了水稻对干旱胁迫的耐受性，降低了发芽率，抑制了干旱处理下的根长。此外，OE系还降低了OE系对外源ABA的敏感性。更重要的是，酵母单杂交实验表明，PhePP2CA13作用于PhebZIP47的下游，双荧光素酶实验进一步表明，PhePP2CA13的表达被PhebZIP47抑制。本研究丰富了对PP2CA基因的认识，为毛竹抗逆性育种提供了遗传资源。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.