Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis

IF 15.8 1区生物学 Q1 PLANT SCIENCES

Nature Plants Pub Date : 2025-04-22 DOI:10.1038/s41477-025-01989-9

Trevor Weiss, Maris Kamalu, Honglue Shi, Zheng Li, Jasmine Amerasekera, Zhenhui Zhong, Benjamin A. Adler, Michelle M. Song, Kamakshi Vohra, Gabriel Wirnowski, Sidharth Chitkara, Charlie Ambrose, Noah Steinmetz, Ananya Sridharan, Diego Sahagun, Jillian F. Banfield, Jennifer A. Doudna, Steven E. Jacobsen

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引用次数: 0

Abstract

Genome editing is transforming plant biology by enabling precise DNA modifications. However, delivery of editing systems into plants remains challenging, often requiring slow, genotype-specific methods such as tissue culture or transformation¹. Plant viruses, which naturally infect and spread to most tissues, present a promising delivery system for editing reagents. However, many viruses have limited cargo capacities, restricting their ability to carry large CRISPR-Cas systems. Here we engineered tobacco rattle virus (TRV) to carry the compact RNA-guided TnpB enzyme ISYmu1 and its guide RNA. This innovation allowed transgene-free editing of Arabidopsis thaliana in a single step, with edits inherited in the subsequent generation. By overcoming traditional reagent delivery barriers, this approach offers a novel platform for genome editing, which can greatly accelerate plant biotechnology and basic research.

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病毒递送rna引导基因组编辑器用于拟南芥无转基因生殖系编辑

基因组编辑通过实现精确的 DNA 修饰，正在改变植物生物学。然而，将编辑系统输送到植物体内仍具有挑战性，通常需要采用组织培养或转化等缓慢、基因型特异的方法1。植物病毒可自然感染并传播到大多数组织，是一种很有前景的编辑试剂输送系统。然而，许多病毒的载货能力有限，限制了它们携带大型 CRISPR-Cas 系统的能力。在这里，我们改造了烟草拨浪鼓病毒（TRV），使其能携带紧凑型 RNA 引导的 TnpB 酶 ISYmu1 及其引导 RNA。通过这一创新技术，我们只需一步就能对拟南芥进行无转基因编辑，并将编辑结果遗传给下一代。这种方法克服了传统试剂传递的障碍，为基因组编辑提供了一个新的平台，可大大加快植物生物技术和基础研究的发展。

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来源期刊

Nature Plants PLANT SCIENCES-

CiteScore

25.30

自引率

2.20%

发文量

196

期刊介绍： Nature Plants is an online-only, monthly journal publishing the best research on plants — from their evolution, development, metabolism and environmental interactions to their societal significance.