Discovery of a novel alphasatellite of the genus Clecrusatellite associated with a wide array of New World tomato-infecting begomoviruses

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-04-22 DOI:10.1007/s00705-025-06293-7

Luciane de Nazaré A. dos Reis, Leonardo S. Boiteux, Maria Esther N. Fonseca, Rita de Cássia Pereira-Carvalho

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引用次数: 0

Abstract

A new alphasatellite was discovered via high-throughput sequencing in mixed infections with five distinct tomato-infecting begomoviruses in central Brazil, and the presence of this alphasatellite in tomato leaf samples was confirmed by PCR and Sanger sequencing. It has a circular genome of 1,321 nucleotides with typical features of alphasatellites belonging to the subfamily Geminialphasatellitinae, including a replication-associated hairpin sequence (TAGTATTAC) and an open reading frame encoding a replication-associated protein. Pairwise sequence comparisons and phylogenetic analysis indicated that this alphasatellite represents a new species, with 69–77% sequence identity to other members of the genus Clecrusatellite. Tomato golden vein virus (TGVV) was the only begomovirus present in all six positive samples. However, it was not possible to determine which of these viruses can function as a helper virus for the alphasatellite. The species name "Clecrusatellite solanumbrasiliensis" is proposed to classify this alphasatellite associated with endemic Brazilian begomoviruses. To our knowledge, this is the first report of a tomato-associated alphasatellite in the New World.

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发现了一种与大量新世界感染番茄的begomovirus相关的新α星属

通过高通量测序，在巴西中部5种不同的番茄侵染begomovirus混合侵染中发现了一种新的alphasatsatellite，并通过PCR和Sanger测序证实了该alphasatsatellite在番茄叶片样本中的存在。它具有1,321个核苷酸的圆形基因组，具有属于Geminialphasatellitinae亚家族的alphasatellitae的典型特征，包括复制相关发夹序列（TAGTATTAC）和编码复制相关蛋白的开放阅读框。序列比对和系统发育分析表明，该alphasatellite为新种，序列与其他Clecrusatellite属成员同源性为69 ~ 77%。6份阳性样品中仅存在番茄金静脉病毒（TGVV）。然而，无法确定这些病毒中的哪一种可以作为阿尔法卫星的辅助病毒。种名“Clecrusatellite solanumbrasiliensis”被提议用来分类这种与巴西地方性begomoviruses相关的alphasatellite。据我们所知，这是在新大陆发现的第一颗与番茄相关的卫星。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.