Liposome of Phlorizin promote the repair of carotid atherosclerosis in rats by regulating inflammation and the Nrf2 signaling pathway

IF 3.9 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2025-04-18 DOI:10.1016/j.bbalip.2025.159613

Xiaoyu Wu , Chuanbo Ding , Xinglong Liu , Qiteng Ding , Shuai Zhang , Yue Wang , Ting Zhao , Wencong Liu

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引用次数: 0

Abstract

Carotid atherosclerosis (CAS) is a complex cardiovascular disease linked to inflammatory response and oxidative stress. This study aimed to develop and assess the therapeutic efficacy of Phlorizin liposomes (Phlorizin-Lips) in repairing CAS in rats. Phlorizin-Lips were prepared using the film dispersion method and evaluated for controlled release, antioxidant properties, and biocompatibility. The methodology included preparing Phlorizin-Lips, conducting in vitro and in vivo experiments, observing histopathological changes in carotid arteries in a rat model, and detecting inflammatory markers and antioxidant gene expression in arterial endothelial cells using immunoblotting and ELISA. Results showed that Phlorizin-Lips significantly lowered inflammatory markers TNF-α and IL-1β in endothelial cells while upregulating Nrf2 and its downstream antioxidant genes, enhancing the cells' antioxidant capacity and reducing oxidative damage by activating the Nrf2 signaling pathway. Additionally, Phlorizin-Lips reduced carotid plaque formation, improved vascular endothelial function, and promoted CAS repair. This study underscores Phlorizin's potential as a therapeutic agent for CAS and highlights the Nrf2 pathway's role in regulating inflammation and oxidative stress. Future research will explore the clinical potential of Phlorizin-Lips.

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根连素脂质体通过调节炎症和Nrf2信号通路促进大鼠颈动脉粥样硬化的修复

颈动脉粥样硬化（CAS）是一种与炎症反应和氧化应激相关的复杂心血管疾病。本研究旨在开发和评价根连素脂质体（根连素唇）修复大鼠CAS的疗效。采用薄膜分散法制备了苯连菌素唇状体，并对其控释、抗氧化性能和生物相容性进行了评价。方法包括制备邻苯二甲酸乙酯，进行体外和体内实验，观察大鼠颈动脉组织病理学变化，利用免疫印迹法和ELISA法检测动脉内皮细胞炎症标志物和抗氧化基因表达。结果显示，Phlorizin-Lips可显著降低内皮细胞炎症标志物TNF-α和IL-1β，上调Nrf2及其下游抗氧化基因，通过激活Nrf2信号通路，增强细胞抗氧化能力，减轻氧化损伤。此外，Phlorizin-Lips减少颈动脉斑块形成，改善血管内皮功能，促进CAS修复。本研究强调了Phlorizin作为CAS治疗药物的潜力，并强调了Nrf2通路在调节炎症和氧化应激中的作用。未来的研究将进一步探索其临床应用潜力。

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来源期刊

Biochimica et biophysica acta. Molecular and cell biology of lipids 生物-生化与分子生物学

CiteScore

11.00

自引率

2.10%

发文量

109

审稿时长

53 days

期刊介绍： BBA Molecular and Cell Biology of Lipids publishes papers on original research dealing with novel aspects of molecular genetics related to the lipidome, the biosynthesis of lipids, the role of lipids in cells and whole organisms, the regulation of lipid metabolism and function, and lipidomics in all organisms. Manuscripts should significantly advance the understanding of the molecular mechanisms underlying biological processes in which lipids are involved. Papers detailing novel methodology must report significant biochemical, molecular, or functional insight in the area of lipids.