Theoretical study of modified multiple dual-mode elution counter-current chromatography based on chiral separation

Abstract

The fact that both phases are liquids makes it simple to implement counter-current chromatography (CCC), which involves alternating elution modes and phase roles during the separation process. Using two distinct multiple dual-mode (MDM) elution techniques for chiral separation, the team demonstrated a superior separation effect in a prior study. When selecting a chiral selector with high enantiomer recognition capability is difficult, the separation efficiency of the CCC column can be enhanced through multiple cycles. This approach facilitates the preparation of enantiomers that are challenging to isolate under classical CCC conditions. A straightforward mathematical model was created in this study to forecast the number of cycles and the mode conversion time needed for the separation process after the modified MDM method was further examined theoretically. After modeling molecules, the theoretical model was confirmed, and the outcomes generally agreed with the calculations from the theoretical model. This offers substantial support for the modified MDM method's real-world implementation in chiral separation.