Harnessing genetic potentials for drought tolerance in wheat (Triticum aestivum L.) using tolerance indices and molecular markers

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-04-16 DOI:10.1016/j.genrep.2025.102230

Mst. Anamika Amzad , Md. Arifuzzaman , Md. Ashraful Alam

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Abstract

Drought is a major abiotic stressor in bread wheat that causes substantial yield losses globally. So, the investigation was conducted to find out drought tolerant genotypes in bread wheat using tolerance indices and molecular characterization at juvenile and grain filling stages. The experiments were conducted with 30 bread wheat genotypes and 54 simple sequence repeats (SSRs) alleles. The experiment was designed as randomized complete block design with three treatments viz. 0 M, 0.2 M, and 0.4 M mannitol solution at juvenile stages and split plot design with two treatments viz. control and drought at grain filling stages. The results revealed significant variations among the genotypes for traits under study in both stages. To reveal drought tolerance among the genotypes, six drought tolerance indices were employed. The genotype BARI Gom 30, BARI Gom 32, BARI Gom 22, BARI Gom 26, BARI Gom 27 and BWMRI-1 carried superior rank score and regarded as drought tolerant genotypes. For molecular genetic characterization, in total 54 alleles were detected using SSRs where number of alleles locus ranged from 4 to 9 with an average of 5.4. The 30 bread wheat genotypes grouped into two populations and four unique clusters. Finally, considering the morpho-physiological and molecular study, the genotypes BARI Gom 22, BARI Gom26, BARI Gom 30 and BARI Gom 32 are identified as drought tolerant genotypes and provide an undertaking aims for further research and breeding efforts to develop wheat varieties with tolerance against drought to subtropical environments.

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利用耐旱指数和分子标记挖掘小麦（Triticum aestivum L.）的耐旱遗传潜力

干旱是面包小麦的主要非生物胁迫因素，在全球范围内造成大量产量损失。因此，本研究通过对面包小麦幼期和灌浆期耐旱性指标和分子特性的分析，确定了面包小麦的耐旱性基因型。采用30个面包小麦基因型和54个简单序列重复（SSRs）等位基因进行试验。试验采用完全随机区组设计，在苗期采用0 M、0.2 M和0.4 M甘露醇溶液3个处理，灌浆期采用对照和干旱2个处理的分割区设计。结果显示，在这两个阶段，所研究性状的基因型之间存在显著差异。为了揭示不同基因型的耐旱性，采用了6个耐旱性指标。基因型BARI Gom 30、BARI Gom 32、BARI Gom 22、BARI Gom 26、BARI Gom 27和BWMRI-1的等级得分较高，被认为是耐旱基因型。在分子遗传鉴定方面，共检测到54个等位基因，等位基因位点数量为4 ~ 9个，平均为5.4个。30个面包小麦基因型分为2个群体和4个独特的群体。最后，结合形态生理和分子生物学研究，确定BARI Gom 22、BARI Gom26、BARI Gom 30和BARI Gom 32基因型为耐干旱基因型，为进一步研究和培育亚热带环境下的耐干旱小麦品种提供了工作目标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.