Regulation of Sugar Metabolism During Fermentation of Brewers' Spent Grain by Leuconostoc pseudomesenteroides DSM20193

Abstract

Re-utilising brewers' spent grain (BSG) through LAB fermentation can enable its broad use in the food industry, enhancing its nutritional and functional properties and offering a clear example of a sustainable approach in the valorisation of food side streams. Despite extensive research on LAB fermentation, the regulation of metabolism during the growth in complex food-industry-relevant environments remains unclear. This study investigates the metabolic processes in Leuconostoc pseudomesenteroides DSM20193 during 24 h fermentation of BSG with and without 4% sucrose (w/w) supplementation, allowing in situ dextran synthesis. Besides dextran synthesis, the presence of sucrose led to faster acidification, especially due to the increased formation of acetic acid. Furthermore, differences in the utilisation of sucrose, fructose, glucose, and maltose and the formation of diverse oligosaccharides were observed. Transcriptome analysis comparing expression profiles during 0 h and 16 h growth in BSG with sucrose revealed differences in the expression of genes involved in carbohydrate utilisation pathways, including higher activity of sucrose and maltose metabolism and lower activity of metabolism related to alternative carbon sources. Transcription analysis of selected relevant genes in a time-course comparison between BSG with and without sucrose provided more detailed indications of responses of the metabolic network in this complex environment. This analysis provided a deeper understanding of the dynamic regulatory mechanism that drives sugar metabolism and dextran synthesis and how the presence of sucrose can alter the metabolic flux towards different fermentation products.