Unraveling the in vitro antioxidant and neuroprotective potential of green amalgamated gold nanoparticles from Chang shuo huang ma (Corchorus olitorius (L)): Insights on SK-N-SH neuroblastoma cell lines

Pharmacological Research - Modern Chinese Medicine Pub Date : 2025-04-15 DOI:10.1016/j.prmcm.2025.100615

Basheerahmed Abdulaziz Mannasaheb , Ibrahim Ahmed Shaikh , Gurmeet Kaur Surindar Singh , Noordin Othman , Mohammed M. Ghoneim , Hanish Singh Jayasingh Chellammal

{"title":"Unraveling the in vitro antioxidant and neuroprotective potential of green amalgamated gold nanoparticles from Chang shuo huang ma (Corchorus olitorius (L)): Insights on SK-N-SH neuroblastoma cell lines","authors":"Basheerahmed Abdulaziz Mannasaheb , Ibrahim Ahmed Shaikh , Gurmeet Kaur Surindar Singh , Noordin Othman , Mohammed M. Ghoneim , Hanish Singh Jayasingh Chellammal","doi":"10.1016/j.prmcm.2025.100615","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>The global healthcare management for neurodegenerative disorders is increasing, mandating the need to discover safe and effective medications to manage them. The plant Chang shuo huang ma, commonly known as <em>Corchorus olitorius</em> (CO) is used in the folk Chinese medicine, thus extensively grown in China. Numerous traditional Chinese medicines (TCM) from natural products have shown beneficial effects in neurodegenerative diseases.</div></div><div><h3>Methods</h3><div>The CO leaves were successively extracted using hexane, ethyl acetate, ethanol and water and subjected to qualitative phytochemical analysis and quantification of phenols and flavonoids. The aqueous extract of CO assisted the green synthesis of gold nanoparticles (Au<sup>0</sup>). The green synthesized gold nanoparticles (CO-AuNP) were characterized by UV–Visible spectroscopy, FTIR, XRD, DLS, SEM and EDX. The CO-AuNP were screened for <em>in vitro</em> anti-inflammatory, antioxidant and neuroprotective potential.</div></div><div><h3>Results</h3><div>The aqueous extract showed presence of phenols, flavonoids, sterols, terpenoids and saponins with higher content of total phenol (3485.60 µg) and flavonoids (2578.44 µg). The UV–Vis spectroscopy showed a strong peak at 560 nm. The FTIR revealed the presence of bioactive functional groups on the surface of CO-AuNP. XRD analysis showed crystalline structure and DLS indicated a particle size of 101.7 nm with moderate stability (-0.3 mV) from zeta potential. The SEM images revealed the presence of irregular shapes of aggregated patterns of CO-AuNP and EDX indicated sufficient composition of elemental gold. The anti-inflammatory activity against heat induced protein denaturation and cyclooxygenase- II (COX-II) inhibition with IC<sub>50</sub> values of 167.06 and 149.46 µg/mL were observed. Antioxidant results were notable in DPPH assay with IC<sub>50</sub> value of 133.25 µg/mL. A dose-dependent cytotoxic effect was observed in normal L929 cell lines, with an IC<sub>50</sub> value of 204.11 µg/mL. Potential neuroprotective effects were observed in SK-N-SH neuroblastoma cell lines against trimethyltin chloride (TMT) induced neurotoxicity. The percentage of neuroprotection decreased with the increasing concentration of CO-AuNP, that is lower concentration (50 µg) has shown highest neuroprotection (86.25 %).</div></div><div><h3>Conclusion</h3><div>The present findings feature the application and effectiveness of CO-AuNP against inflammation, oxidative and neuronal damage observed in numerous diseases including neurodegeneration, diabetes and cancer.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"15 ","pages":"Article 100615"},"PeriodicalIF":0.0000,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacological Research - Modern Chinese Medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667142525000442","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Background

The global healthcare management for neurodegenerative disorders is increasing, mandating the need to discover safe and effective medications to manage them. The plant Chang shuo huang ma, commonly known as Corchorus olitorius (CO) is used in the folk Chinese medicine, thus extensively grown in China. Numerous traditional Chinese medicines (TCM) from natural products have shown beneficial effects in neurodegenerative diseases.

Methods

The CO leaves were successively extracted using hexane, ethyl acetate, ethanol and water and subjected to qualitative phytochemical analysis and quantification of phenols and flavonoids. The aqueous extract of CO assisted the green synthesis of gold nanoparticles (Au⁰). The green synthesized gold nanoparticles (CO-AuNP) were characterized by UV–Visible spectroscopy, FTIR, XRD, DLS, SEM and EDX. The CO-AuNP were screened for in vitro anti-inflammatory, antioxidant and neuroprotective potential.

Results

The aqueous extract showed presence of phenols, flavonoids, sterols, terpenoids and saponins with higher content of total phenol (3485.60 µg) and flavonoids (2578.44 µg). The UV–Vis spectroscopy showed a strong peak at 560 nm. The FTIR revealed the presence of bioactive functional groups on the surface of CO-AuNP. XRD analysis showed crystalline structure and DLS indicated a particle size of 101.7 nm with moderate stability (-0.3 mV) from zeta potential. The SEM images revealed the presence of irregular shapes of aggregated patterns of CO-AuNP and EDX indicated sufficient composition of elemental gold. The anti-inflammatory activity against heat induced protein denaturation and cyclooxygenase- II (COX-II) inhibition with IC₅₀ values of 167.06 and 149.46 µg/mL were observed. Antioxidant results were notable in DPPH assay with IC₅₀ value of 133.25 µg/mL. A dose-dependent cytotoxic effect was observed in normal L929 cell lines, with an IC₅₀ value of 204.11 µg/mL. Potential neuroprotective effects were observed in SK-N-SH neuroblastoma cell lines against trimethyltin chloride (TMT) induced neurotoxicity. The percentage of neuroprotection decreased with the increasing concentration of CO-AuNP, that is lower concentration (50 µg) has shown highest neuroprotection (86.25 %).

Conclusion

The present findings feature the application and effectiveness of CO-AuNP against inflammation, oxidative and neuronal damage observed in numerous diseases including neurodegeneration, diabetes and cancer.

查看原文本刊更多论文

揭示常朔黄麻绿色汞化金纳米颗粒的体外抗氧化和神经保护潜力：SK-N-SH神经母细胞瘤细胞系的见解

背景神经退行性疾病的全球医疗保健管理正在增加，要求需要发现安全有效的药物来管理它们。常朔黄麻，俗称Corchorus olitorius (CO)，在民间中药中使用，因此在中国广泛种植。许多来自天然产物的传统中药（TCM）已显示出对神经退行性疾病的有益作用。方法分别用正己烷、乙酸乙酯、乙醇和水提取CO叶，对其酚类和黄酮类进行定性分析和定量分析。CO的水提物有助于绿色合成金纳米颗粒（Au0）。采用紫外可见光谱、FTIR、XRD、DLS、SEM和EDX对绿色合成金纳米粒子（CO-AuNP）进行了表征。对CO-AuNP进行体外抗炎、抗氧化和神经保护作用的筛选。结果水提物中含有酚类、黄酮类、甾醇类、萜类和皂苷类成分，总酚（3485.60µg）和总黄酮（2578.44µg）含量较高。紫外可见光谱在560nm处有一个强峰。FTIR显示CO-AuNP表面存在生物活性官能团。XRD和DLS分析表明，该材料的晶粒尺寸为101.7 nm， zeta电位稳定（-0.3 mV）。SEM图像显示CO-AuNP和EDX的聚集模式呈不规则形状，表明元素金的成分充足。抗热诱导蛋白变性和抑制环氧合酶-II （COX-II）的IC50值分别为167.06和149.46µg/mL。DPPH抗氧化效果显著，IC50值为133.25µg/mL。对正常L929细胞株有剂量依赖性的细胞毒作用，IC50值为204.11µg/mL。SK-N-SH神经母细胞瘤细胞株对三甲基氯化锡（TMT）诱导的神经毒性具有潜在的神经保护作用。神经保护作用随CO-AuNP浓度的增加而降低，以浓度较低（50µg）的神经保护作用最强（86.25%）。结论CO-AuNP在神经退行性疾病、糖尿病和癌症等多种疾病中具有抗炎症、抗氧化和抗神经元损伤的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Pharmacological Research - Modern Chinese Medicine

CiteScore

1.60

自引率

0.00%

发文量