Chemical composition, α-amylase inhibition, and antioxidant activities of Scabiosa hololeuca Bornm.

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology Pub Date : 2025-04-20 DOI:10.1016/j.bse.2025.105028

Kübra Öğüt , Gülmira Özek , Nilgün Öztürk , Ömer Koray Yaylacı , Temel Özek

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引用次数: 0

Abstract

Scabiosa hololeuca Bornm., a plant from the Caprifoliaceae family, grows naturally in the Kütahya-Eskişehir region of Türkiye. This study investigates the chemical composition and biological activities of S. hololeuca extracts. The plant was subjected to various extraction methods, including essential oil, fatty acids, n-hexane, methanol, and water extracts. The GC-FID/MS technique was employed to analyze the chemical composition of essential oil and fatty acids. In the hydrodistilled essential oil, dodecanoic acid (11.000 ± 0.0015 %) and tetradecanoic acid (10.592 ± 0.033 %) were identified as the major volatile compounds. Volatile compounds in the leaves and flowers were extracted via the MSD-SPME technique, with alloaromadendrene (24.908 ± 0.033 %) as the predominant volatile in the leaves and naphthalene (14.106 ± 0.045 %) in the flowers. Lipophilic compounds, such as methyl nonadecanoate (44.903 ± 0.001 %) and methyl hexadecanoate (41.299 ± 0.011 %), were the main fatty acids in the leaves and flowers, respectively. The antioxidant potential of the extracts was evaluated through various assays, including DPPH• and ABTS+• free radical scavenging, lipid peroxidation inhibition (β-carotene bleaching), and ORAC. The methanol extract exhibited the highest antioxidant activity, with the strongest DPPH• scavenging activity (IC₅₀ 0.24 ± 0.01 mg/mL) and significant β-carotene inhibition (IC₅₀ 1.3 ± 0.02 mg/mL). The water extract showed the highest ORAC values. However, S. hololeuca extracts did not show effective α-amylase inhibition. The RP-HPLC analysis revealed o-coumaric and caffeic acids as major phenolic components in the methanol extract. These findings suggest potential industrial applications of S. hololeuca due to its rich chemical profile and antioxidant properties.

查看原文本刊更多论文

斑点疥螨的化学成分、α-淀粉酶抑制作用及抗氧化活性。

蝎尾草（Scabiosa hololeuca Bornm.）是一种卷叶菜科植物，自然生长在土耳其的库塔希亚-埃斯基谢希尔地区。本研究调查了 S. hololeuca 提取物的化学成分和生物活性。该植物采用了多种萃取方法，包括精油、脂肪酸、正己烷、甲醇和水萃取。采用 GC-FID/MS 技术分析了精油和脂肪酸的化学成分。在水蒸馏精油中，十二酸（11.000 ± 0.0015 %）和十四酸（10.592 ± 0.033 %）被确定为主要的挥发性化合物。通过 MSD-SPME 技术提取叶片和花朵中的挥发性化合物，发现叶片中最主要的挥发性化合物是异戊二烯（24.908 ± 0.033 %），花朵中最主要的挥发性化合物是萘酚（14.106 ± 0.045 %）。亲脂化合物，如十一酸甲酯（44.903 ± 0.001 %）和十六酸甲酯（41.299 ± 0.011 %），分别是叶和花中的主要脂肪酸。萃取物的抗氧化潜力通过各种检测方法进行了评估，包括 DPPH 和 ABTS+-自由基清除、脂质过氧化抑制（β-胡萝卜素漂白）和 ORAC。甲醇提取物的抗氧化活性最高，DPPH-清除活性最强（IC50 0.24 ± 0.01 mg/mL），对β-胡萝卜素的抑制作用也很明显（IC50 1.3 ± 0.02 mg/mL）。水提取物的 ORAC 值最高。然而，S. hololeuca 提取物并未显示出有效的 α 淀粉酶抑制作用。RP-HPLC 分析显示，甲醇提取物中的主要酚类成分是邻香豆酸和咖啡酸。这些研究结果表明，S. hololeuca 具有丰富的化学成分和抗氧化特性，具有潜在的工业应用价值。

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来源期刊

Biochemical Systematics and Ecology 生物-进化生物学

CiteScore

3.00

自引率

12.50%

发文量

147

审稿时长

43 days

期刊介绍： Biochemical Systematics and Ecology is devoted to the publication of original papers and reviews, both submitted and invited, in two subject areas: I) the application of biochemistry to problems relating to systematic biology of organisms (biochemical systematics); II) the role of biochemistry in interactions between organisms or between an organism and its environment (biochemical ecology). In the Biochemical Systematics subject area, comparative studies of the distribution of (secondary) metabolites within a wider taxon (e.g. genus or family) are welcome. Comparative studies, encompassing multiple accessions of each of the taxa within their distribution are particularly encouraged. Welcome are also studies combining classical chemosystematic studies (such as comparative HPLC-MS or GC-MS investigations) with (macro-) molecular phylogenetic studies. Studies that involve the comparative use of compounds to help differentiate among species such as adulterants or substitutes that illustrate the applied use of chemosystematics are welcome. In contrast, studies solely employing macromolecular phylogenetic techniques (gene sequences, RAPD studies etc.) will be considered out of scope. Discouraged are manuscripts that report known or new compounds from a single source taxon without addressing a systematic hypothesis. Also considered out of scope are studies using outdated and hard to reproduce macromolecular techniques such as RAPDs in combination with standard chemosystematic techniques such as GC-FID and GC-MS.