Surgery-related change in cancer cell adhesion associates with recurrence in patients undergoing colorectal cancer surgery

IF 3.5 2区医学 Q2 ONCOLOGY

Ejso Pub Date : 2025-04-15 DOI:10.1016/j.ejso.2025.110055

Stine Bull Jessen , Rasmus Peuliche Vogelsang , Troels Gammeltoft Dolin , Jannie Jørgensen , Josephine Bjergbæk Olsson , Tove Kirkegaard , Ismail Gögenur , Jesper Thorvald Troelsen

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引用次数: 0

Abstract

Objectives

This study aimed to investigate the effect of pre- and postoperative serum on the adhesion of cultured colon cancer cells and their relationship with colorectal cancer recurrence.

Background

Colorectal cancer is common, and surgery is the primary treatment choice. However, surgical procedures may be associated with an increased risk of recurrence.

Method

434 patients undergoing curatively intended colorectal cancer surgery at Copenhagen University Hospital, Herlev, Denmark, between July 15, 2014, and March 31, 2019, were included in the study. Pre- and postoperative serum samples were collected, and the effect on cellular adhesion was analyzed using a novel high-throughput approach based on CRISPR/Cas9 modified Caco-2 cells and secreted luciferase, named the AdhesionScore assay. The relative risk of postoperative recurrence was estimated using Cox proportional regression analysis.

Results

The difference in adhesion between modified Caco-2 cells seeded in the pre- and postoperative serum showed a significant increase in postoperative adhesion in patients with a recurrence event (p=0.0293). Modeling the adhesion data using multiple logistic regression and Cox proportional regression analyses showed a statistically significant association between increased postoperative adhesion and recurrence (p=0.0155 and p=0.0126, respectively). Patients with the highest AdhesionScore showed the greatest risk of recurrence (HR=7, 95% CI 1.6-37.8, p=0.0130).

Conclusions

The study found that a difference in the adhesion of Caco-2 cells seeded in pre- and postoperative serum was associated with cancer recurrence following intended curative surgery. This suggests that increased postoperative adhesion may serve as a novel biological marker of recurrence in patients undergoing surgery for colorectal cancer.

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结直肠癌手术患者手术相关的癌细胞粘附改变与复发相关

目的探讨术前和术后血清对培养结肠癌细胞黏附的影响及其与结直肠癌复发的关系。结直肠癌很常见，手术是主要的治疗选择。然而，外科手术可能会增加复发的风险。方法纳入2014年7月15日至2019年3月31日期间在丹麦Herlev的哥本哈根大学医院接受治愈性结直肠癌手术的434例患者。收集术前和术后血清样本，采用基于CRISPR/Cas9修饰的Caco-2细胞和分泌的荧光素酶的新型高通量方法（称为粘连评分法）分析对细胞粘附的影响。采用Cox比例回归分析估计术后复发的相对风险。结果术前和术后血清中植入改性Caco-2细胞的黏附差异显示，复发患者术后黏附显著增加（p=0.0293）。使用多重逻辑回归和Cox比例回归分析对粘连数据进行建模，结果显示术后粘连增加与复发之间存在统计学意义的关联（p=0.0155和p=0.0126）。粘连评分最高的患者复发风险最高（HR=7, 95% CI 1.6 ~ 37.8, p=0.0130）。结论研究发现，术前和术后血清中Caco-2细胞黏附的差异与预期治愈性手术后的癌症复发有关。这表明，术后粘连增加可能是结直肠癌手术患者复发的一种新的生物学标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ejso 医学-外科

CiteScore

6.40

自引率

2.60%

发文量

1148

审稿时长

41 days

期刊介绍： JSO - European Journal of Surgical Oncology ("the Journal of Cancer Surgery") is the Official Journal of the European Society of Surgical Oncology and BASO ~ the Association for Cancer Surgery. The EJSO aims to advance surgical oncology research and practice through the publication of original research articles, review articles, editorials, debates and correspondence.