A near-infrared fluorescent probe with a self-immolative linker for rapid detection of beta-galactosidase and specific imaging of ovarian cancer cells

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal Pub Date : 2025-04-14 DOI:10.1016/j.microc.2025.113640

Fu Shu , Zhihui Cheng , Gang Yuan , Yuan Qiu , Qi Sun , Hanping He , Genyan Liu

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引用次数: 0

Abstract

Beta-galactosidase (β-gal) is a vital biological target which is widely used in the diagnosis of ovarian cancers. To date, only a few near-infrared fluorescent probes were successfully applied to monitor β-gal activity both in vitro and in vivo. However, these β-gal probes have disadvantages as poor water-solubility or slow response rate. To overcome these shortcomings, we constructed a novel near-infrared fluorescent probe (Cy-βgal) for β-gal tracing by using self-immolative group connected with fluorophore hemicyanine skeleton (Cy-OH) and recognition group (D-galactose). As expected, Cy-βgal displayed a fast response rate (8 min) to β-gal in water solution containing only 0.1 % DMSO with a fluorescent turn-on model. In addition, Cy-βgal exhibited favorable affinity (K_m = 23.51 μM) to β-gal accompanied with high catalytic efficiency (k_cat/K_m = 2.47 μM⁻¹ s⁻¹). Most importantly, Cy-βgal was able to specifically distinguish the ovarian cancer cells from lung cancer cells via the red fluorescence imaging signal of endogenous β-gal in living cells, implying that Cy-βgal has the potential for the diagnosis of ovarian cancer. It also confirmed that the self-immolative group-based designing strategy is a practical method for the rational design of other types of β-gal fluorescent probes.

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来源期刊

Microchemical Journal 化学-分析化学

CiteScore

8.70

自引率

8.30%

发文量

1131

审稿时长

1.9 months

期刊介绍： The Microchemical Journal is a peer reviewed journal devoted to all aspects and phases of analytical chemistry and chemical analysis. The Microchemical Journal publishes articles which are at the forefront of modern analytical chemistry and cover innovations in the techniques to the finest possible limits. This includes fundamental aspects, instrumentation, new developments, innovative and novel methods and applications including environmental and clinical field. Traditional classical analytical methods such as spectrophotometry and titrimetry as well as established instrumentation methods such as flame and graphite furnace atomic absorption spectrometry, gas chromatography, and modified glassy or carbon electrode electrochemical methods will be considered, provided they show significant improvements and novelty compared to the established methods.