Holo-omics disentangle drought response and biotic interactions among plant, endophyte and pathogen

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2025-04-18 DOI:10.1111/nph.70155

Peilin Chen, Qingyi Yu, Cong Wang, Liliam Montoya, Patrick T. West, Ling Xu, Nelle Varoquaux, Benjamin Cole, Kim K. Hixson, Young-Mo Kim, Ling Liu, Baodan Zhang, Jie Zhang, Baiyang Li, Elizabeth Purdom, John Vogel, Christer Jansson, Robert B. Hutmacher, Jeffery A. Dahlberg, Devin Coleman-Derr, Peggy G. Lemaux, John W. Taylor, Cheng Gao

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引用次数: 0

Abstract

Holo-omics provide a novel opportunity to study the interactions among fungi from different functional guilds in host plants in field conditions.
We address the entangled responses of plant pathogenic and endophytic fungi associated with sorghum when droughted through the assembly of the most abundant fungal, endophyte genome from rhizospheric metagenomic sequences followed by a comparison of its metatranscriptome with the host plant metabolome and transcriptome.
The rise in relative abundance of endophytic Acremonium persicinum (operational taxonomic unit 5 (OTU5)) in drought co-occurs with a rise in fungal membrane dynamics and plant metabolites, led by ethanolamine, a key phospholipid membrane component. The negative association between endophytic A. persicinum (OTU5) and plant pathogenic fungi co-occurs with a rise in expression of the endophyte's biosynthetic gene clusters coding for secondary compounds. Endophytic A. persicinum (OTU5) and plant pathogenic fungi are negatively associated under preflowering drought but not under postflowering drought, likely a consequence of variation in fungal fitness responses to changes in the availability of water and niche space caused by plant maturation over the growing season.
Our findings suggest that the dynamic biotic interactions among host, beneficial and harmful microbiota in a changing environment can be disentangled by a blending of field observation, laboratory validation, holo-omics and ecological modelling.

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全息组学揭示了植物、内生菌和病原体之间的干旱响应和生物相互作用

全息组学为在田间条件下研究寄主植物中不同功能行会真菌之间的相互作用提供了新的机会。我们通过从根际宏基因组序列中组装最丰富的真菌、内生真菌基因组，并将其与寄主植物代谢组和转录组进行比较，来解决与高粱相关的植物病原真菌和内生真菌在干旱时的纠缠反应。在干旱条件下，内生真菌Acremonium persicinum（操作分类单元5 (OTU5)）相对丰度的增加与真菌膜动力学和植物代谢物的增加同时发生，以乙醇胺（磷脂膜的关键成分）为主导。内生菌a . persicinum （OTU5）与植物病原真菌之间的负相关与内生菌编码次生化合物的生物合成基因簇表达的增加同时发生。开花前干旱与植物致病真菌呈负相关，开花后干旱与植物致病真菌呈负相关，这可能是由于植物成熟期引起的水分和生态位空间变化导致真菌适应度变化的结果。我们的研究结果表明，在不断变化的环境中，宿主、有益和有害微生物群之间的动态生物相互作用可以通过实地观察、实验室验证、全息组学和生态模型的混合来解开。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.