{"title":"Electrokinetic Chromatography-based Micro Methods for Separation and Physiochemical Characterization of Very Hydrophobic Pharmaceuticals","authors":"Robert Minkner, Hermann Wätzig","doi":"10.1002/jssc.70127","DOIUrl":null,"url":null,"abstract":"<p>This study established an efficient separation of the highly hydrophobic analytes fenofibrate, orlistat, lumefantrine and a micelle marker using microemulsion electrokinetic capillary chromatography. A starting buffer with sodium dodecyl sulphate, heptane and butan-1-ol resulted in a partial separation of the analytes. Various experimental parameters were investigated, including voltage, sample loading time, buffer composition and temperature. An excellent separation was achieved, and compounds with hydrophobicity of up to a calculated log <i>P</i> of 10.2 (est. 12) could be clearly distinguished. Due to observed variabilities in retention time when changing the capillaries, it is advisable to calibrate with a standard mixture before each measurement series.</p>","PeriodicalId":17098,"journal":{"name":"Journal of separation science","volume":"48 4","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.70127","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of separation science","FirstCategoryId":"5","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jssc.70127","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
This study established an efficient separation of the highly hydrophobic analytes fenofibrate, orlistat, lumefantrine and a micelle marker using microemulsion electrokinetic capillary chromatography. A starting buffer with sodium dodecyl sulphate, heptane and butan-1-ol resulted in a partial separation of the analytes. Various experimental parameters were investigated, including voltage, sample loading time, buffer composition and temperature. An excellent separation was achieved, and compounds with hydrophobicity of up to a calculated log P of 10.2 (est. 12) could be clearly distinguished. Due to observed variabilities in retention time when changing the capillaries, it is advisable to calibrate with a standard mixture before each measurement series.
期刊介绍：
The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.
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