Effect of physical modification on the immunomodulatory activity of carboxymethyl pachymaran

IF 8.5 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-04-17 DOI:10.1016/j.ijbiomac.2025.143253

Zhaoxi Chen , Yalin Zhao , Xi Feng , Lijia Zhang , Xiaoju Tian , Salam A. Ibrahim , Wen Huang , Ying Liu

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引用次数: 0

Abstract

Carboxymethyl pachymaran (CMP) was modified by high temperature (HT), high pressure (HP) and gamma irradiation (GI) to obtain HT-CMP, HP-CMP, and GI-CMP samples, respectively. The effects of different modification methods on the immunomodulatory activity of CMPs were evaluated by cell culture in vitro and animal in vivo tests. The modified CMPs showed a stronger immunomodulatory activity than that of CMP in vitro. The modified CMPs improved the proliferative and phagocytic capacities, the ROS release, the TNF-α cytokine secretion of RAW264.7 compared with the CMP. For in vivo test, we found that the CMP and modified CMPs were able to enhance the immune function in mice with cyclophosphamide (CTX) induced by immunosuppression. All the CMPs could increase the cytokine levels (IL-2, IL-6, IFN-γ, TNF-α) and elevate the mRNA expression of NF-κB and TLRs. Furthermore, CMP, HT-CMP and GI-CMP had an ameliorative effect on intestinal flora disorders and could restore the richness and diversity of intestinal flora. Our results provided a theoretical foundation for the potential development of CMP-based immunomodulatory drugs.

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物理修饰对羧甲基茯苓多糖免疫调节活性的影响

采用高温（HT）、高压（HP）和γ辐照（GI）对羧甲基茯苓多糖（CMP）进行改性，分别得到HT-CMP、HP-CMP和GI-CMP样品。通过体外细胞培养和动物体内实验，评价不同修饰方法对中药多糖免疫调节活性的影响。与体外实验相比，修饰后的CMP具有更强的免疫调节活性。与CMP相比，改良的CMP提高了RAW264.7的增殖和吞噬能力，ROS释放，TNF-α细胞因子分泌。在体内实验中，我们发现CMP和修饰的CMP能够增强环磷酰胺（CTX）免疫抑制小鼠的免疫功能。各组细胞因子IL-2、IL-6、IFN-γ、TNF-α水平升高，NF-κB、TLRs mRNA表达升高。此外，CMP、HT-CMP和GI-CMP对肠道菌群紊乱有改善作用，可以恢复肠道菌群的丰富度和多样性。本研究结果为潜在开发基于cmp的免疫调节药物提供了理论基础。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.