Microbial inoculant accelerated pig slurry maturation at low-temperature anaerobic digestion: Performance and mechanism

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2025-04-12 DOI:10.1016/j.biortech.2025.132532

Yali Yang , Zhe Zhao , Dengchao Lei , Hongbo He , Hongtu Xie , Xudong Zhang , Shijun Sun , Xuelian Bao , Yulan Zhang

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Abstract

This study investigated the impact of microbial inoculants on maturing pig slurry during anaerobic digestion (AD) at psychrophilic temperatures (10 °C). A simulation experiment was conducted with two treatments: pig slurry alone (CK) and pig slurry supplemented with microbial inoculants (MA). The MA treatment achieved faster maturity than CK, as evidenced by an improved germination index (achieving 70 % in advance) and a reduced content of organic pollutants, which was attributed to an increased relative abundance of hydrolytic microorganisms (7–21 %) and decreased pathogens (6–9 %) at the early stage (<42 (d)). Microbial inoculants influenced pig slurry microorganisms by serving as keystone taxa (B_OTU229 (f_Ruminococcaceae) and F_OTU76 (s_Acremonium alcalophilum)), and stimulating new keystone taxa (B_OTU423 (g_NK4A214) and F_OTU25 (g_Clavulina)) through associative interactions, thereby intensifying saprotrophic and carbon cycling processes, and accelerating maturation. These results provide a biological strategy for maturing pig slurry and help elucidate the mechanisms underlying microbial effects.

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微生物接种剂在低温厌氧消化条件下加速猪浆液成熟：性能和机理

本研究研究了微生物接种剂对在厌氧消化（AD）过程中成熟的猪浆在亲湿温度（10℃）的影响。采用猪浆单独（CK）和猪浆中添加微生物接种剂（MA）两种处理进行了模拟试验。与CK相比，MA处理的成熟速度更快，萌发指数提高（提前70%），有机污染物含量降低，这是由于早期水解微生物相对丰度增加（7 - 21%），病原体相对丰度减少（6 - 9%）（<42 (d)）。微生物接种剂对猪浆微生物的影响主要表现为：作为关键分类群（B_OTU229 （f_Ruminococcaceae）和F_OTU76 (s_Acremonium alcalophilum)），并通过关联相互作用刺激新的关键分类群（B_OTU423 （g_NK4A214）和F_OTU25 (g_Clavulina)），从而强化腐养和碳循环过程，加速成熟。这些结果为成熟猪浆提供了生物学策略，并有助于阐明微生物作用的机制。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.