Strategies for acquiring electrochemiluminescence spectra and their applications in mechanism studies and multi-analyte determination

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-04-14 DOI:10.1016/j.trac.2025.118259

Lixin Zang , Haiyan Song , Huimin Zhao , Qiao Zhang , Qi Kang , Dazhong Shen

{"title":"Strategies for acquiring electrochemiluminescence spectra and their applications in mechanism studies and multi-analyte determination","authors":"Lixin Zang , Haiyan Song , Huimin Zhao , Qiao Zhang , Qi Kang , Dazhong Shen","doi":"10.1016/j.trac.2025.118259","DOIUrl":null,"url":null,"abstract":"<div><div>Owing to its high sensitivity and close-to-zero background, the electrochemiluminescence (ECL) technique is widely used in biological analysis, environmental detection, and other fields. ECL spectra can provide molecular-level information useful for elucidating ECL mechanisms and expanding the scope of ECL applications. However, acquiring ECL spectra is challenging, as the ECL intensity changes over measurement time. Herein, we review three strategies for ECL spectrum acquisition, namely, the combinations of (i) a photomultiplier tube detector used in single-wavelength mode with repeated ECL excitation, (ii) a charge-coupled device detector array and monochromator with a single ECL excitation scan, and (iii) a photomultiplier tube detector and band-pass filter array turntable with a single ECL excitation scan based on ECL intensity correction. Subsequently, we discuss the applications of ECL spectroscopy, including luminescence mechanism investigations, spectrum-resolved multianalyte determination, and ratiometric ECL sensors.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"189 ","pages":"Article 118259"},"PeriodicalIF":11.8000,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Trends in Analytical Chemistry","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S016599362500127X","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Owing to its high sensitivity and close-to-zero background, the electrochemiluminescence (ECL) technique is widely used in biological analysis, environmental detection, and other fields. ECL spectra can provide molecular-level information useful for elucidating ECL mechanisms and expanding the scope of ECL applications. However, acquiring ECL spectra is challenging, as the ECL intensity changes over measurement time. Herein, we review three strategies for ECL spectrum acquisition, namely, the combinations of (i) a photomultiplier tube detector used in single-wavelength mode with repeated ECL excitation, (ii) a charge-coupled device detector array and monochromator with a single ECL excitation scan, and (iii) a photomultiplier tube detector and band-pass filter array turntable with a single ECL excitation scan based on ECL intensity correction. Subsequently, we discuss the applications of ECL spectroscopy, including luminescence mechanism investigations, spectrum-resolved multianalyte determination, and ratiometric ECL sensors.

查看原文本刊更多论文

获取电化学发光光谱的策略及其在机理研究和多分析物测定中的应用

电化学发光（ECL）技术以其高灵敏度和接近零的本底特性，广泛应用于生物分析、环境检测等领域。ECL光谱为阐明ECL机制和扩大ECL的应用范围提供了分子水平的信息。然而，获取ECL光谱具有挑战性，因为ECL强度随测量时间而变化。在此，我们回顾了三种ECL光谱采集策略，即(i)在单波长模式下使用具有重复ECL激发的光电倍增管探测器，（ii）电荷耦合器件探测器阵列和单色仪与单ECL激发扫描的组合，以及（iii）光电倍增管探测器和带通滤波器阵列转台与基于ECL强度校正的单ECL激发扫描的组合。随后，我们讨论了ECL光谱的应用，包括发光机理研究、光谱分辨多分析物测定和比例ECL传感器。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.