A novel quantitative evaluation strategy of polysaccharides from Lycium barbarum L. fruits based on simultaneous quantification of total polysaccharides and polysaccharides with active structure characteristics

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2025-04-16 DOI:10.1016/j.indcrop.2025.121023

Baojie Zhu , Wenxia Zhang , Shaoping Li , Jing Zhao

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引用次数: 0

Abstract

The polysaccharides from L. barbarum L. fruits (LBPs) are a kind of complex polysaccharides, which mainly contribute to the bioactivity of L. barbarum L. fruits and riches in acidic polysaccharides fractions. And the pharmacological research showed that the immunomodulatory effect of LBPs was closely related to α-1,4-D-galactosiduronic linkages. Therefore, as one of the main active polysaccharide fractions in LBPs, acidic polysaccharides fraction should also be used for the quality control of L. barbarum L. fruits. In this study, a novel quantitative evaluation strategy of polysaccharides from L. barbarum L. fruits based on simultaneous quantification of total polysaccharides and polysaccharides with active structure characteristics was proposed to improve their performance in the medicinal and functional food area more comprehensively and effectively, and beneficial to improve the quality control of L. barbarum L. fruits. The content of total polysaccharides in L. barbarum L. fruits was determined by HPSEC-RID method combined with universal dn/dc (0.15 mL·g⁻¹). Additionally, HPAEC-PAD method combined with pectinase hydrolysis and m-hydroxybiphenyl colorimetric method were developed for the determination of acidic polysaccharide components related to active structural characteristics in L. barbarum fruits. The results revealed that the trends of total polysaccharides and acidic polysaccharides in L. barbarum L. fruits did not entirely coincide, further emphasizing the necessity of simultaneously quantifying both fractions for quality control. Furthermore, the m-hydroxybiphenyl method can be used as an alternative method to determine the content of acidic polysaccharides by HPAEC-PAD combined with pectinase hydrolysis.

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基于总多糖和活性结构特征多糖同时定量的枸杞多糖定量评价新策略

枸杞果实多糖（LBPs）是一种复合多糖，主要参与枸杞果实的生物活性，富含酸性多糖组分。药理研究表明，lbp的免疫调节作用与α-1,4- d -半乳糖醛酸键密切相关。因此，酸性多糖作为枸杞多糖的主要活性组分之一，也可用于枸杞果实的质量控制。本研究提出了一种基于总多糖和活性结构特征多糖同时定量的枸杞多糖定量评价新策略，以更全面有效地提高枸杞多糖在药用和功能食品领域的性能，有利于提高枸杞果实的质量控制。采用通用dn/dc（0.15 mL·g−1）联合hplc - rid法测定枸杞果实中总多糖的含量。此外，还建立了HPAEC-PAD联合果胶酶水解法和间羟基联苯比色法测定枸杞果实中与活性结构特征相关的酸性多糖成分。结果表明，枸杞果实中总多糖和酸性多糖的变化趋势并不完全一致，进一步强调了同时定量测定两种组分的必要性。此外，间羟基联苯法可作为HPAEC-PAD联合果胶酶水解测定酸性多糖含量的替代方法。

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.