A Novel Technology to Boost Natural Production of Hyaluronic Acid in the Skin Tissue: Human Histology Study

IF 2.3 4区医学 Q2 DERMATOLOGY

Journal of Cosmetic Dermatology Pub Date : 2025-04-17 DOI:10.1111/jocd.70159

David J. Goldberg

{"title":"A Novel Technology to Boost Natural Production of Hyaluronic Acid in the Skin Tissue: Human Histology Study","authors":"David J. Goldberg","doi":"10.1111/jocd.70159","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Introduction</h3>\n \n <p>Combining monopolar radiofrequency (RF) with targeted ultrasound (TUS), this study investigated whether these modalities promote facial rejuvenation through the production of hyaluronic acid (HA) in human skin.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Seven subjects (51–64 years, BMI 21.1–29.8 kg/m<sup>2</sup>) were enrolled and divided into three treatment groups in this single-center study; Group A (<i>n</i> = 3, simultaneous RF + TUS), Group B (<i>n</i> = 3, stand-alone RF), and control (<i>n</i> = 1, no treatment). Both treated groups underwent four (4) 60-min treatments on the face delivered 7–14 days apart. Punch biopsies (3 mm in diameter) were collected from the infra-auricular area at baseline and both follow-up visits and stained for HA by using hyaluronic acid binding protein. Digital photographs were taken to document changes in visual appearance. Finally, the subjects' comfort and satisfaction were assessed.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>There was a statistically significant (<i>p</i> < 0.05) average increase at 1 month in the HA-stained area of +112 358.7 μm<sup>2</sup> in group A (RF + TUS) representing an increase of 48.65%. The treatment effect peaked at 3 months with an increase of +156 345.2 μm<sup>2</sup>, corresponding to a 67.69% increase in the HA-stained area. In Group B, there was no significant difference in the average increase of the HA-stained area between 1 month (+14 830 μm<sup>2</sup>) and 3 months (+20 995 μm<sup>2</sup>) corresponding to a 6.76% and 9.56% increase, respectively. The control samples did not indicate any changes throughout the study. Digital photographs of the RF + TUS group showed both a decrease in rhytids and tighter skin. Therapies were comfortable with no adverse events.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Overall, this study has shown that the combination treatment of RF + TUS has a more pronounced and sustained effect on facial rejuvenation compared to RF alone. The measurable increase in the production of HA with simultaneous use of RF + TUS peaked at a 3-month follow-up, suggesting the gradual advancement of the treatment effect and overall improvement in facial appearance.</p>\n </section>\n \n <section>\n \n <h3> Trial Registration</h3>\n \n <p>ClinicalTrials.gov identifier: NCT05987917</p>\n </section>\n </div>","PeriodicalId":15546,"journal":{"name":"Journal of Cosmetic Dermatology","volume":"24 4","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jocd.70159","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cosmetic Dermatology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jocd.70159","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DERMATOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction

Combining monopolar radiofrequency (RF) with targeted ultrasound (TUS), this study investigated whether these modalities promote facial rejuvenation through the production of hyaluronic acid (HA) in human skin.

Methods

Seven subjects (51–64 years, BMI 21.1–29.8 kg/m²) were enrolled and divided into three treatment groups in this single-center study; Group A (n = 3, simultaneous RF + TUS), Group B (n = 3, stand-alone RF), and control (n = 1, no treatment). Both treated groups underwent four (4) 60-min treatments on the face delivered 7–14 days apart. Punch biopsies (3 mm in diameter) were collected from the infra-auricular area at baseline and both follow-up visits and stained for HA by using hyaluronic acid binding protein. Digital photographs were taken to document changes in visual appearance. Finally, the subjects' comfort and satisfaction were assessed.

Results

There was a statistically significant (p < 0.05) average increase at 1 month in the HA-stained area of +112 358.7 μm² in group A (RF + TUS) representing an increase of 48.65%. The treatment effect peaked at 3 months with an increase of +156 345.2 μm², corresponding to a 67.69% increase in the HA-stained area. In Group B, there was no significant difference in the average increase of the HA-stained area between 1 month (+14 830 μm²) and 3 months (+20 995 μm²) corresponding to a 6.76% and 9.56% increase, respectively. The control samples did not indicate any changes throughout the study. Digital photographs of the RF + TUS group showed both a decrease in rhytids and tighter skin. Therapies were comfortable with no adverse events.

Conclusion

Overall, this study has shown that the combination treatment of RF + TUS has a more pronounced and sustained effect on facial rejuvenation compared to RF alone. The measurable increase in the production of HA with simultaneous use of RF + TUS peaked at a 3-month follow-up, suggesting the gradual advancement of the treatment effect and overall improvement in facial appearance.

Trial Registration

ClinicalTrials.gov identifier: NCT05987917

Abstract Image

查看原文本刊更多论文

促进皮肤组织中透明质酸自然生成的新技术：人类组织学研究

本研究结合单极射频（RF）和靶向超声（TUS），研究了这些方式是否通过人体皮肤中透明质酸（HA）的产生来促进面部恢复活力。方法7例51 ~ 64岁，体重指数21.1 ~ 29.8 kg/m2的受试者分为3个治疗组；A组（n = 3，同时射频+ TUS）， B组（n = 3，单独射频）和对照组（n = 1，不治疗）。两组均接受4次面部治疗，每次60分钟，间隔7-14天。在基线和两次随访时从耳下区域收集穿孔活检（直径3mm），并使用透明质酸结合蛋白进行HA染色。数码照片记录了视觉外观的变化。最后，评估受试者的舒适度和满意度。结果a组（RF + TUS） ha染色面积+112 358.7 μm2, 1个月平均增加48.65%，差异有统计学意义（p < 0.05）。治疗效果在第3个月达到顶峰，增加了+156 345.2 μm2， ha染色面积增加了67.69%。B组ha染色面积在1个月（+14 830 μm2）和3个月（+20 995 μm2）间的平均增幅无显著差异，分别为6.76%和9.56%。对照样本在整个研究过程中没有任何变化。RF + TUS组的数码照片显示心律失常减少，皮肤紧致。治疗过程舒适，无不良事件发生。总的来说，本研究表明，与单独使用RF相比，RF + TUS联合治疗在面部年轻化方面具有更明显和持续的效果。同时使用RF + TUS可测量的HA生成增加在随访3个月时达到峰值，表明治疗效果逐渐进步，面部外观整体改善。试验注册ClinicalTrials.gov标识符：NCT05987917

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Cosmetic Dermatology DERMATOLOGY-

CiteScore

4.30

自引率

13.00%

发文量

818

审稿时长

>12 weeks

期刊介绍： The Journal of Cosmetic Dermatology publishes high quality, peer-reviewed articles on all aspects of cosmetic dermatology with the aim to foster the highest standards of patient care in cosmetic dermatology. Published quarterly, the Journal of Cosmetic Dermatology facilitates continuing professional development and provides a forum for the exchange of scientific research and innovative techniques. The scope of coverage includes, but will not be limited to: healthy skin; skin maintenance; ageing skin; photodamage and photoprotection; rejuvenation; biochemistry, endocrinology and neuroimmunology of healthy skin; imaging; skin measurement; quality of life; skin types; sensitive skin; rosacea and acne; sebum; sweat; fat; phlebology; hair conservation, restoration and removal; nails and nail surgery; pigment; psychological and medicolegal issues; retinoids; cosmetic chemistry; dermopharmacy; cosmeceuticals; toiletries; striae; cellulite; cosmetic dermatological surgery; blepharoplasty; liposuction; surgical complications; botulinum; fillers, peels and dermabrasion; local and tumescent anaesthesia; electrosurgery; lasers, including laser physics, laser research and safety, vascular lasers, pigment lasers, hair removal lasers, tattoo removal lasers, resurfacing lasers, dermal remodelling lasers and laser complications.