Comparative phytochemical profiling and authentication of four Artemisia species using integrated GC-MS, HPTLC and NIR spectroscopy approach

IF 4.3 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

BMC Chemistry Pub Date : 2025-04-16 DOI:10.1186/s13065-025-01467-5

Ingy I. Abdallah, Hebaalla A. Mahmoud, Nadia A. El-Sebakhy, Yasmin A. Mahgoub

{"title":"Comparative phytochemical profiling and authentication of four Artemisia species using integrated GC-MS, HPTLC and NIR spectroscopy approach","authors":"Ingy I. Abdallah, Hebaalla A. Mahmoud, Nadia A. El-Sebakhy, Yasmin A. Mahgoub","doi":"10.1186/s13065-025-01467-5","DOIUrl":null,"url":null,"abstract":"<div><p>Genus <i>Artemisia</i> has diverse phytochemistry and a long history in traditional medicine with several species still having unexplored potential. Hence, comparative profiling of <i>Artemisia</i> species in Egypt (<i>A. annua</i>, <i>A. herba-alba</i>, <i>A. monosperma</i> and <i>A. judaica</i>) and their authentication is of great interest. An integrated approach of GC-MS, HPTLC-image analysis and near-infrared (NIR) spectroscopy was implemented for their fingerprinting, discrimination and authentication. GC-MS analysis revealed the phytochemical profile of their volatile oils identifying compounds spanning monoterpenes, sesquiterpenes, diterpenes and non-terpenoid compounds. The major chemical components were highlighted as camphor, β-caryophyllene and germacrene D in <i>A. annua</i>, camphene, cis-pinocarveol, trans-chrysanthenyl acetate and cis-chrysanthenyl acetate in <i>A. herba-alba</i>, α-pinene, β-pinene, α-terpinolene and (-)-spathulenol in <i>A. monosperma</i>, finally, camphor, piperitone and trans-ethyl cinnamate in <i>A. judaica</i>. HPTLC-image analysis allowed tracking chemical markers in their total alcoholic extracts. Artemisinin was detected only in <i>A. annua</i> while scopoletin was identified as a major characteristic coumarin in <i>Artemisia</i> species. Phenolic acids and flavonoids were also discovered in the different species. Finally, NIR spectroscopy allowed profiling and authentication of their powders revealing prominent spectral characteristics correlated to the chemical markers identified by GC-MS and HPTLC. Then, multivariate analysis facilitated classification and discrimination of the species. Additionally, PLS regression analysis was utilized for quality control of powdered <i>A. annua</i>, being an important industrial crop, by detecting its adulteration with other species in limits of detection less than 1.5%. This combined approach aided in the rapid comparative profiling of the <i>Artemisia</i> species as a mean for their fingerprinting and authentication.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":496,"journal":{"name":"BMC Chemistry","volume":"19 1","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bmcchem.biomedcentral.com/counter/pdf/10.1186/s13065-025-01467-5","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Chemistry","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1186/s13065-025-01467-5","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Genus Artemisia has diverse phytochemistry and a long history in traditional medicine with several species still having unexplored potential. Hence, comparative profiling of Artemisia species in Egypt (A. annua, A. herba-alba, A. monosperma and A. judaica) and their authentication is of great interest. An integrated approach of GC-MS, HPTLC-image analysis and near-infrared (NIR) spectroscopy was implemented for their fingerprinting, discrimination and authentication. GC-MS analysis revealed the phytochemical profile of their volatile oils identifying compounds spanning monoterpenes, sesquiterpenes, diterpenes and non-terpenoid compounds. The major chemical components were highlighted as camphor, β-caryophyllene and germacrene D in A. annua, camphene, cis-pinocarveol, trans-chrysanthenyl acetate and cis-chrysanthenyl acetate in A. herba-alba, α-pinene, β-pinene, α-terpinolene and (-)-spathulenol in A. monosperma, finally, camphor, piperitone and trans-ethyl cinnamate in A. judaica. HPTLC-image analysis allowed tracking chemical markers in their total alcoholic extracts. Artemisinin was detected only in A. annua while scopoletin was identified as a major characteristic coumarin in Artemisia species. Phenolic acids and flavonoids were also discovered in the different species. Finally, NIR spectroscopy allowed profiling and authentication of their powders revealing prominent spectral characteristics correlated to the chemical markers identified by GC-MS and HPTLC. Then, multivariate analysis facilitated classification and discrimination of the species. Additionally, PLS regression analysis was utilized for quality control of powdered A. annua, being an important industrial crop, by detecting its adulteration with other species in limits of detection less than 1.5%. This combined approach aided in the rapid comparative profiling of the Artemisia species as a mean for their fingerprinting and authentication.

Graphical Abstract

查看原文本刊更多论文

采用气相色谱-质谱、高效液相色谱和近红外光谱综合分析方法比较四种青蒿的植物化学特征和鉴定

青蒿属植物化学成分多样，在传统医学中有着悠久的历史，有几个品种仍有未开发的潜力。因此，对埃及青蒿（A. annua， A. herba-alba， A. monosperma和A. judaica）的比较分析及其鉴定具有重要意义。采用气相色谱-质谱、hptlc -图像分析和近红外（NIR）光谱相结合的方法对其进行指纹识别、鉴别和鉴定。GC-MS分析揭示了其挥发油的植物化学特征，鉴定出单萜、倍半萜、二萜和非萜类化合物。主要化学成分为黄花蒿中的樟脑、β-石竹烯和肉桂烯D，白花蒿中的樟脑、顺式蒎醇、反式乙酸大黄烯和顺式乙酸大黄烯，单精蒿中的α-蒎烯、β-蒎烯、α-松油烯和(-)-鸡皮酚，黄樟中的樟脑、胡椒酮和肉桂酸反式乙酯。hptlc图像分析允许跟踪其总酒精提取物中的化学标记。青蒿素仅在黄花蒿中检测到，而东莨菪素是黄花蒿中主要的香豆素。在不同的种类中也发现了酚酸和类黄酮。最后，通过近红外光谱对其粉末进行分析和鉴定，揭示了与GC-MS和HPTLC鉴定的化学标记相关的显著光谱特征。然后，通过多变量分析促进了物种的分类和鉴别。此外，利用PLS回归分析方法对重要工业作物黄花蒿粉进行了质量控制，检测出其与其他品种的掺假，检出限小于1.5%。这种综合方法有助于对青蒿类植物进行快速比较分析，作为指纹和鉴定的一种手段。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

BMC Chemistry Chemistry-General Chemistry

CiteScore

5.30

自引率

2.20%

发文量

审稿时长

27 weeks

期刊介绍： BMC Chemistry, formerly known as Chemistry Central Journal, is now part of the BMC series journals family. Chemistry Central Journal has served the chemistry community as a trusted open access resource for more than 10 years – and we are delighted to announce the next step on its journey. In January 2019 the journal has been renamed BMC Chemistry and now strengthens the BMC series footprint in the physical sciences by publishing quality articles and by pushing the boundaries of open chemistry.