Parallel Tapered Optical Fiber Biosensor for Highly Sensitive Detection of Mucin 1

IF 4.3 2区综合性期刊 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

IEEE Sensors Journal Pub Date : 2025-03-11 DOI:10.1109/JSEN.2025.3547840

Chu Chu;Xinyu Yang;Haili Jiang;Xinghua Yang;Minghua Ma;Pingping Teng;Shengjia Wang;Rui Wang;Xingyue Wen;Kang Li;Bo Zhang;Adam Jones;Qianqing Yu

{"title":"Parallel Tapered Optical Fiber Biosensor for Highly Sensitive Detection of Mucin 1","authors":"Chu Chu;Xinyu Yang;Haili Jiang;Xinghua Yang;Minghua Ma;Pingping Teng;Shengjia Wang;Rui Wang;Xingyue Wen;Kang Li;Bo Zhang;Adam Jones;Qianqing Yu","doi":"10.1109/JSEN.2025.3547840","DOIUrl":null,"url":null,"abstract":"We built a reflective parallel tapered optical fiber structure and used it as a biosensor for highly sensitive in situ detection of label-free Mucin 1 (MUC1). A sensing tapered optical fiber probe was prepared, and a reference probe of similar free spectral range (FSR) was connected in parallel to establish the optical Vernier effect. This arrangement amplified the refractive index (RI) sensitivity to 8.4 times that of a single sensing probe with 19845.22 nm/RIU. Meanwhile, the aptamer was chemically bonded to the surface of sensing probe to specifically detect MUC1. This biosensor has a linear wide detection range from <inline-formula> <tex-math>$10^{-{10}}$ </tex-math></inline-formula> to <inline-formula> <tex-math>$10^{-{1}}$ </tex-math></inline-formula> mg/mL, with a high sensitivity of 12.56 nm/log(C/C0) and a low limit of detection (LOD) of 0.012 pg/mL. Significantly, the reflective structures, unlike transmissive ones, could be inserted into samples rather than requiring samples to be moved to the sensing area, which not only reduces the contamination of samples during movement but also enables the measurement of trace samples that cannot be transferred. Furthermore, this novel sensing structure with high sensitivity holds promise for the preliminary diagnosis and prognostic monitoring of cancer and has broad development prospects in clinical and therapeutic applications.","PeriodicalId":447,"journal":{"name":"IEEE Sensors Journal","volume":"25 8","pages":"13027-13032"},"PeriodicalIF":4.3000,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"IEEE Sensors Journal","FirstCategoryId":"103","ListUrlMain":"https://ieeexplore.ieee.org/document/10919022/","RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}

引用次数: 0

Abstract

We built a reflective parallel tapered optical fiber structure and used it as a biosensor for highly sensitive in situ detection of label-free Mucin 1 (MUC1). A sensing tapered optical fiber probe was prepared, and a reference probe of similar free spectral range (FSR) was connected in parallel to establish the optical Vernier effect. This arrangement amplified the refractive index (RI) sensitivity to 8.4 times that of a single sensing probe with 19845.22 nm/RIU. Meanwhile, the aptamer was chemically bonded to the surface of sensing probe to specifically detect MUC1. This biosensor has a linear wide detection range from

$10^{-{10}}$

$10^{-{1}}$

mg/mL, with a high sensitivity of 12.56 nm/log(C/C0) and a low limit of detection (LOD) of 0.012 pg/mL. Significantly, the reflective structures, unlike transmissive ones, could be inserted into samples rather than requiring samples to be moved to the sensing area, which not only reduces the contamination of samples during movement but also enables the measurement of trace samples that cannot be transferred. Furthermore, this novel sensing structure with high sensitivity holds promise for the preliminary diagnosis and prognostic monitoring of cancer and has broad development prospects in clinical and therapeutic applications.

查看原文本刊更多论文

用于高灵敏度检测粘蛋白 1 的平行锥形光纤生物传感器

我们构建了一种反射平行锥形光纤结构，并将其作为生物传感器用于高灵敏度的原位检测无标签粘蛋白1 （MUC1）。制备了传感锥形光纤探头，并将自由光谱范围相近的参考探头并联，建立了光游标效应。这种排列将折射率（RI）灵敏度提高到单个传感探针（19845.22 nm/RIU）的8.4倍。同时，该适体与传感探针表面化学键合，特异检测MUC1。该传感器线性宽检测范围为$10^{-{1}}$ ~ $10^{-{1}}$ mg/mL，灵敏度为12.56 nm/log(C/C0)，低检出限（LOD）为0.012 pg/mL。值得注意的是，与透射结构不同，反射结构可以插入样品中，而不需要将样品移动到传感区域，这不仅减少了样品在移动过程中的污染，而且可以测量无法转移的痕量样品。此外，这种具有高灵敏度的新型传感结构有望对癌症进行初步诊断和预后监测，在临床和治疗应用中具有广阔的发展前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

IEEE Sensors Journal 工程技术-工程：电子与电气

CiteScore

7.70

自引率

14.00%

发文量

2058

审稿时长

5.2 months

期刊介绍： The fields of interest of the IEEE Sensors Journal are the theory, design , fabrication, manufacturing and applications of devices for sensing and transducing physical, chemical and biological phenomena, with emphasis on the electronics and physics aspect of sensors and integrated sensors-actuators. IEEE Sensors Journal deals with the following: -Sensor Phenomenology, Modelling, and Evaluation -Sensor Materials, Processing, and Fabrication -Chemical and Gas Sensors -Microfluidics and Biosensors -Optical Sensors -Physical Sensors: Temperature, Mechanical, Magnetic, and others -Acoustic and Ultrasonic Sensors -Sensor Packaging -Sensor Networks -Sensor Applications -Sensor Systems: Signals, Processing, and Interfaces -Actuators and Sensor Power Systems -Sensor Signal Processing for high precision and stability (amplification, filtering, linearization, modulation/demodulation) and under harsh conditions (EMC, radiation, humidity, temperature); energy consumption/harvesting -Sensor Data Processing (soft computing with sensor data, e.g., pattern recognition, machine learning, evolutionary computation; sensor data fusion, processing of wave e.g., electromagnetic and acoustic; and non-wave, e.g., chemical, gravity, particle, thermal, radiative and non-radiative sensor data, detection, estimation and classification based on sensor data) -Sensors in Industrial Practice