CyanoStat: An open-source platform for CO2 regulation in microbial incubators optimized for cyanobacterial cultivation

IF 2 Q3 ENGINEERING, ELECTRICAL & ELECTRONIC

HardwareX Pub Date : 2025-04-12 DOI:10.1016/j.ohx.2025.e00649

Tijn O. Delzenne, Dennis Claessen

{"title":"CyanoStat: An open-source platform for CO2 regulation in microbial incubators optimized for cyanobacterial cultivation","authors":"Tijn O. Delzenne, Dennis Claessen","doi":"10.1016/j.ohx.2025.e00649","DOIUrl":null,"url":null,"abstract":"<div><div>Accurate and reliable control of CO<sub>2</sub> is essential for the incubation of many microbial organisms. Photosynthetic microbial organisms, such as cyanobacteria, pose a particular challenge, requiring both light and elevated CO<sub>2</sub> concentrations for efficient growth. These characteristics highly limit the availability of affordable and reliable incubation devices, deterring laboratories from undergoing proof-of-concept research into cyanobacteria. To combat this, this study presents CyanoStat: an affordable add-on device for off-the-shelf microbial incubators. By consistently measuring the CO<sub>2</sub> concentrations in an incubator, control is achieved by opening and closing the flow of gas from a pressurized bottle to the incubator. Through a tuned time-based valve control system, the device is able to rival the accuracy and stability of significantly more expensive hardware. Multiple implemented safety features allow for reliable usage over prolonged periods. CyanoStat, as described in this work, was able to achieve precise CO<sub>2</sub> control with fluctuations of 120 ppm, 145 ppm and 260 ppm around a setpoint of 1, 2 and 5%, respectively. This demonstrates that the system is well-suited for both short-term proof-of-concept studies and long-term cultivation applications.</div></div>","PeriodicalId":37503,"journal":{"name":"HardwareX","volume":"22 ","pages":"Article e00649"},"PeriodicalIF":2.0000,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"HardwareX","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2468067225000276","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}

引用次数: 0

Abstract

Accurate and reliable control of CO₂ is essential for the incubation of many microbial organisms. Photosynthetic microbial organisms, such as cyanobacteria, pose a particular challenge, requiring both light and elevated CO₂ concentrations for efficient growth. These characteristics highly limit the availability of affordable and reliable incubation devices, deterring laboratories from undergoing proof-of-concept research into cyanobacteria. To combat this, this study presents CyanoStat: an affordable add-on device for off-the-shelf microbial incubators. By consistently measuring the CO₂ concentrations in an incubator, control is achieved by opening and closing the flow of gas from a pressurized bottle to the incubator. Through a tuned time-based valve control system, the device is able to rival the accuracy and stability of significantly more expensive hardware. Multiple implemented safety features allow for reliable usage over prolonged periods. CyanoStat, as described in this work, was able to achieve precise CO₂ control with fluctuations of 120 ppm, 145 ppm and 260 ppm around a setpoint of 1, 2 and 5%, respectively. This demonstrates that the system is well-suited for both short-term proof-of-concept studies and long-term cultivation applications.

Abstract Image

查看原文本刊更多论文

CyanoStat：为蓝藻培养优化的微生物培养箱中的CO2调节的开源平台

准确可靠的CO2控制对许多微生物的孵化至关重要。光合微生物，如蓝藻，构成了一个特别的挑战，需要光和高二氧化碳浓度才能有效地生长。这些特点极大地限制了可负担得起和可靠的孵化设备的可用性，阻碍了实验室对蓝藻进行概念验证研究。为了解决这个问题，本研究提出了CyanoStat：一种可负担得起的现成微生物培养器附加设备。通过持续测量培养箱中的二氧化碳浓度，通过打开和关闭从加压瓶到培养箱的气体流来实现控制。通过调谐的基于时间的阀门控制系统，该设备能够与更昂贵的硬件的精度和稳定性相媲美。多个实现的安全功能允许长时间可靠使用。在这项工作中，CyanoStat能够实现精确的CO2控制，分别在设定点1、2和5%左右，波动为120 ppm、145 ppm和260 ppm。这表明该系统非常适合短期的概念验证研究和长期的种植应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

HardwareX Engineering-Industrial and Manufacturing Engineering

CiteScore

4.10

自引率

18.20%

发文量

124

审稿时长

24 weeks

期刊介绍： HardwareX is an open access journal established to promote free and open source designing, building and customizing of scientific infrastructure (hardware). HardwareX aims to recognize researchers for the time and effort in developing scientific infrastructure while providing end-users with sufficient information to replicate and validate the advances presented. HardwareX is open to input from all scientific, technological and medical disciplines. Scientific infrastructure will be interpreted in the broadest sense. Including hardware modifications to existing infrastructure, sensors and tools that perform measurements and other functions outside of the traditional lab setting (such as wearables, air/water quality sensors, and low cost alternatives to existing tools), and the creation of wholly new tools for either standard or novel laboratory tasks. Authors are encouraged to submit hardware developments that address all aspects of science, not only the final measurement, for example, enhancements in sample preparation and handling, user safety, and quality control. The use of distributed digital manufacturing strategies (e.g. 3-D printing) is encouraged. All designs must be submitted under an open hardware license.