Magnetite/reduced graphene oxide composites: A sustainable strategy for selenium immobilization and improved corn growth

Abstract

This study investigated the effectiveness of magnetite/reduced graphene oxide (MRGO) in immobilizing selenium (Se) in contaminated soils and its impact on corn (Zea mays) growth and nutrient uptake. A factorial experiment was conducted in a completely randomized design with three replications under greenhouse conditions. Soil samples were contaminated with sodium selenate at Se concentrations of 0, 2, 4, 8, 16, and 32 mg/kg, equilibrated for two months, and subsequently treated with MRGO at 0, 0.25, 0.5, and 1 %, followed by a three-month incubation period. Corn plants were then cultivated from the seedling stage to the vegetative phase, and growth parameters, along with Se and nutrient concentrations in roots and shoots, were analyzed. MRGO significantly enhanced Se immobilization, increasing retention from 62–71 % to 82–90 % at 1 % MRGO. Se exhibited a biphasic effect on plant growth, promoting growth at 2 mg/kg but inducing toxicity at concentrations exceeding 4 mg/kg, leading to reduced biomass and nutrient uptake. MRGO mitigated Se toxicity by lowering Se accumulation in roots and shoots. However, in Se-free soils, higher MRGO levels negatively affected plant growth, likely due to nutrient adsorption. Trends in potassium (K), iron (Fe), and zinc (Zn) mirrored plant growth, peaking at 2 mg/kg Se and 1 % MRGO, whereas P (P) exhibited an inverse root-shoot distribution. These findings underscore MRGO's potential for Se immobilization in contaminated soils, improving plant performance at optimal Se levels. However, its application in Se-deficient soils requires careful management to prevent nutrient depletion (P, K, Zn, and Fe) and long-term declines in soil fertility.