Exochorda racemosa attenuates DSS-induced colitis in C57BL/6 J mice by regulating inflammatory factors, reducing oxidative stress, and modulating intestinal flora

IF 6.7 1区医学 Q1 CHEMISTRY, MEDICINAL

Phytomedicine Pub Date : 2025-04-13 DOI:10.1016/j.phymed.2025.156768

Sha Long , Lu Wang , Qi Zeng , Yaoyao Li , Jiangtao Su , Yuxin Chen , Gao Zhou

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Abstract

Background

Exochorda racemosa is a member of the genus Exochorda in the Rosaceae family. Its tender leaves and buds are favored as a unique wild vegetable by people in central China.

Purpose

This study systematically evaluated the pharmacological safety and anti-inflammatory efficacy of E. racemosa extracts, with concurrent identification and characterization of their primary bioactive components.

Methods

The chemical composition of E. racemosa extract (ERE) was analyzed using HPLC and LC-MS techniques. The safety and in vivo anti-inflammatory effects of ERE were evaluated using the maximum tolerated dose (MTD) in ICR mice and a dextran sodium sulfate-induced ulcerative colitis model in C57BL/6 J mice.

Results

HPLC and LC-MS analyses revealed that ERE contained abundant flavonoid active ingredients. MTD study confirmed that ERE exhibited good safety. The symptoms of persistent weight loss, DAI, and shortened colon length in UC mice were suppressed by ERE. Pathological damage in colon tissues was attenuated by ERE, with a considerable reduction in histopathological scores and a substantial increase in the number of goblet cells. The levels of IL-6, IL-1β, and TNF-α in serum were significantly decreased following ERE treatment. Moreover, nitric oxide (NO) levels and myeloperoxidase (MPO) activity in colon tissues decreased, whereas glutathione (GSH) levels and superoxide dismutase (SOD) activity in colon tissues increased after ERE treatment. Furthermore, ERE could regulate the intestinal microbial composition and maintain intestinal flora homeostasis, thereby inhibiting inflammatory responses.

Conclusion

ERE exhibited a favorable safety profile and alleviated UC through multiple mechanisms. It is expected to serve as a promising low-toxicity natural product for adjuvant treatment in UC.

Abstract Image

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总状外索虫通过调节炎症因子、降低氧化应激和调节肠道菌群，减轻dss诱导的C57BL/6 J小鼠结肠炎

背景：总形索达（dexochorda racemosa）是蔷蔷科外索达属的一员。它的嫩叶和嫩芽作为一种独特的野生蔬菜受到中国中部人们的青睐。目的系统评价总状叶提取物的药理安全性和抗炎作用，同时对其主要生物活性成分进行鉴定和表征。方法采用高效液相色谱（HPLC）和质谱联用（LC-MS）技术对总状花提取物（ERE）的化学成分进行分析。采用ICR小鼠的最大耐受剂量（MTD）和C57BL/6 J小鼠的葡聚糖硫酸钠诱导的溃疡性结肠炎模型来评价ERE的安全性和体内抗炎作用。结果hplc和LC-MS分析显示，ERE中含有丰富的黄酮类活性成分。MTD研究证实ERE具有良好的安全性。UC小鼠持续体重减轻、DAI和结肠长度缩短的症状被ERE抑制。ERE减轻了结肠组织的病理损伤，组织病理学评分显著降低，杯状细胞数量显著增加。ERE治疗后血清IL-6、IL-1β、TNF-α水平均显著降低。此外，ERE治疗后，结肠组织中一氧化氮（NO）水平和髓过氧化物酶（MPO）活性降低，而结肠组织中谷胱甘肽（GSH）水平和超氧化物歧化酶（SOD）活性升高。此外，ERE还能调节肠道微生物组成，维持肠道菌群稳态，从而抑制炎症反应。结论ere具有良好的安全性，可通过多种机制缓解UC。它有望作为一种有前途的低毒天然产物用于UC的辅助治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytomedicine 医学-药学

CiteScore

10.30

自引率

5.10%

发文量

670

审稿时长

91 days

期刊介绍： Phytomedicine is a therapy-oriented journal that publishes innovative studies on the efficacy, safety, quality, and mechanisms of action of specified plant extracts, phytopharmaceuticals, and their isolated constituents. This includes clinical, pharmacological, pharmacokinetic, and toxicological studies of herbal medicinal products, preparations, and purified compounds with defined and consistent quality, ensuring reproducible pharmacological activity. Founded in 1994, Phytomedicine aims to focus and stimulate research in this field and establish internationally accepted scientific standards for pharmacological studies, proof of clinical efficacy, and safety of phytomedicines.