Dynamic biodistribution of inhaled silica particles to extrapulmonary sites: Early and late translocation mechanisms with implication for particle biomonitoring

IF 10.3 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environment International Pub Date : 2025-04-15 DOI:10.1016/j.envint.2025.109473

Riccardo Leinardi , Chiara Longo Sanchez-Calero , Saloua Ibouraadaten , Francine Uwambayinema , Yousof Yakoub , Cristina Pavan , Rani Claus , Frauke Lemaire , Steven Ronsmans , Manosij Ghosh , Lénárd Farczádi , Horatiu Moldovan , Jeroen A.J. Vanoirbeek , Francesco Turci , Peter H.M. Hoet , François Huaux

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Abstract

An innovative method based on inductively coupled plasma mass spectrometry (ICP-MS) was developed to quantify the time-dependent systemic redistribution pattern of pulmonary-deposited crystalline silica particles by measuring silicon (Si) levels in the lungs, distal organs, and biological fluids. The method was applied in a murine model and validated in blood and urine samples from two occupationally exposed cohorts (miners and porcelain industry workers). In mice, 30 % of silica particles deposited in the lungs via oropharyngeal administration accumulated in extrapulmonary sites in less than 4 months. An early translocation (within 3 days) resulted in silica distribution to liver and kidneys (13 %), followed by a delayed migration (up to 60 days) in mediastinal lymph nodes (12 %), spleen (1.7 %), and abdominal skin (1.7 %). The long-term increase of Si in urine suggested silica renal clearance. Our data also indicated that the toxic potential of particles is a key determinant of extrapulmonary redistribution. The interest of Si as biomarker of exposure has been confirmed in workers exposed to crystalline silica dust. In these individuals, elevated Si levels in blood and urine paralleled silica exposure. Our findings quantify the dynamics of silica biodistribution in extrapulmonary organs, offering new insights on the biomonitoring of silica exposure in different scenarios.

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吸入二氧化硅颗粒在肺外部位的动态生物分布：早期和晚期易位机制与颗粒生物监测的含义

一种基于电感耦合等离子体质谱（ICP-MS）的创新方法被开发出来，通过测量肺、远端器官和生物体液中的硅（Si）水平来量化肺部沉积的结晶二氧化硅颗粒的时间依赖性全身再分布模式。该方法应用于小鼠模型，并在两个职业暴露队列（矿工和陶瓷工业工人）的血液样本和尿液中进行了验证。在小鼠中，30% %的二氧化硅颗粒通过口咽给药沉积在肺内，在不到4 个月内积聚在肺外部位。早期移位（3 天内）导致二氧化硅分布到肝脏和肾脏（13 %），随后延迟迁移（长达60 天）到纵隔淋巴结（12 %）、脾脏（1.7 %）和腹部皮肤（1.7 %）。尿中硅的长期升高提示肾清除二氧化硅。我们的数据还表明，颗粒的毒性潜力是肺外再分布的关键决定因素。硅作为暴露的生物标志物的兴趣已被证实在工人暴露于结晶二氧化硅粉尘。在这些个体中，尿液和血液中硅含量升高与二氧化硅暴露平行。我们的研究结果量化了肺外器官中二氧化硅生物分布的动态，为不同情况下二氧化硅暴露的生物监测提供了新的见解。

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来源期刊

Environment International 环境科学-环境科学

CiteScore

21.90

自引率

3.40%

发文量

734

审稿时长

2.8 months

期刊介绍： Environmental Health publishes manuscripts focusing on critical aspects of environmental and occupational medicine, including studies in toxicology and epidemiology, to illuminate the human health implications of exposure to environmental hazards. The journal adopts an open-access model and practices open peer review. It caters to scientists and practitioners across all environmental science domains, directly or indirectly impacting human health and well-being. With a commitment to enhancing the prevention of environmentally-related health risks, Environmental Health serves as a public health journal for the community and scientists engaged in matters of public health significance concerning the environment.