A Sprayable Hydrogel Based on Biomimetic Polypeptide-Modified Lipid Nanoparticles for Treating Non-Compressible Hemorrhaging

IF 27.4 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Advanced Materials Pub Date : 2025-04-16 DOI:10.1002/adma.202500908

Yajun Feng, Zixuan Zhang, Wenjing Yan, Zhuo Zhang, Yang Li, Chenhui Zhu, Daidi Fan

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Abstract

Non-compressible hemorrhaging is the main cause of death in modern warfare. A biomimetic peptide-modified lipid nanoparticle-based sprayable hydrogel is developed to mimic and amplify the blood coagulation process for effective hemostasis. A platelet adhesion peptide (PAP, sequence: GFOGER) and platelet crosslinking peptide (PCP, sequence: GGQQLK) are customized and conjugated to 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N[methoxy (polyethylene glycol) 2000] acid (DSPE-PEG2000-COOH) via amido bonds to form DSPE-PEG-PAP and DSPE-PEG-PCP, respectively. These compounds are mixed with distearoyl-sn-glycero-3-phosphocholine, cholesterol, and distearoyl-sn-glycero-3-phospho-L-serine (DSPS) to construct the peptide-modified lipid nanoparticles via thin film rehydration. The nanoparticles are incorporated into a CaCl₂-sodium alginate sprayable hydrogel crosslinked via ionic bonds. The application of the hydrogel solution quickly gels and seals the wound. The PAP activates and adheres platelets, the DSPS and Ca²⁺ amplify prothrombin activation, and PCP strengthens the fibrin network. The hydrogel achieves rapid hemostasis within 30 s in a liver hemorrhage model. This sprayable hydrogel has significant potential for managing non-compressible hemorrhaging.

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基于仿生多肽修饰脂质纳米颗粒的可喷雾水凝胶治疗不可压缩性出血

不可压缩性出血是现代战争中造成死亡的主要原因。一种基于仿生肽修饰脂质纳米颗粒的可喷雾水凝胶，用于模拟和放大血液凝固过程，以实现有效止血。定制血小板粘附肽（PAP，序列：GFOGER）和血小板交联肽（PCP，序列：GGQQLK），并通过酰胺键分别与1,2-二硬脂酰-sn-甘油-3-磷酸乙醇胺- n[甲氧基（聚乙二醇）2000]酸（DSPE-PEG2000-COOH）偶联，形成DSPE-PEG-PAP和DSPE-PEG-PCP。这些化合物与二硬脂酰-sn-甘油-3-磷酸胆碱、胆固醇和二硬脂酰-sn-甘油-3-磷酸- l-丝氨酸（DSPS）混合，通过薄膜补液构建肽修饰的脂质纳米颗粒。纳米颗粒通过离子键交联加入到氯化钙-海藻酸钠喷雾水凝胶中。水凝胶溶液的应用迅速凝胶化并密封伤口。PAP激活并粘附血小板，DSPS和Ca2+增强凝血酶原的激活，PCP增强纤维蛋白网络。在肝出血模型中，水凝胶可在30秒内实现快速止血。这种可喷雾的水凝胶在处理不可压缩出血方面具有重要的潜力。

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来源期刊

Advanced Materials 工程技术-材料科学：综合

CiteScore

43.00

自引率

4.10%

发文量

2182

审稿时长

2 months

期刊介绍： Advanced Materials, one of the world's most prestigious journals and the foundation of the Advanced portfolio, is the home of choice for best-in-class materials science for more than 30 years. Following this fast-growing and interdisciplinary field, we are considering and publishing the most important discoveries on any and all materials from materials scientists, chemists, physicists, engineers as well as health and life scientists and bringing you the latest results and trends in modern materials-related research every week.