Nucleic acid-based reference materials: A “ruler” for precision molecular diagnostics

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-04-13 DOI:10.1016/j.trac.2025.118268

Kai Li , Yaning Guo , Xin Qi , Fukai Li , Xinran Li , Mengrui Yang , Jian Zhou , Haiwei Zhou , Gang Liu , Liang Li

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Abstract

Analytical chemistry is experiencing a growing demand for reliable and traceable results, which have significant implications for public health and food safety. The COVID-19 pandemic further underscores the need for accurate and dependable molecular diagnostics. Nucleic acid-based reference materials (NARMs) play a crucial role in ensuring the quality of results. Notably, NARMs have gained increased attention following the outbreak of COVID-19. While previous reviews introduced NARMs but overlooked their applications in different fields. Moreover, the integration of multiple methods represents a new trend in the preparation of NARMs, but these have not appeared in previous reviews. Therefore, this review delineates NARMs classification, synthesizes characterization techniques, reviews their applications in virus diagnosis, animal diseases, pathogenic microorganisms, genetic diseases and genetically modified organism detection, and provides a perspective on NARMs’ development and current challenges. In-depth study of NARMs will be significant for precise molecular diagnostics in biology, agriculture and public health.

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基于核酸的标准物质：精密分子诊断的“标尺”

分析化学对可靠和可追溯结果的需求日益增长，这对公共卫生和食品安全具有重大影响。2019冠状病毒病大流行进一步强调了准确可靠的分子诊断的必要性。核酸基标准物质（NARMs）在保证检测结果质量方面起着至关重要的作用。值得注意的是，在2019冠状病毒病爆发后，narm得到了越来越多的关注。虽然以前的综述介绍了narm，但忽略了它们在不同领域的应用。此外，多种方法的融合是narm制备的新趋势，但在以往的综述中尚未出现。因此，本文综述了narm的分类、综合表征技术，综述了其在病毒诊断、动物疾病、病原微生物、遗传病和转基因生物检测等方面的应用，并对narm的发展和面临的挑战进行了展望。深入研究narm对生物学、农业和公共卫生领域的精确分子诊断具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.