Bacterial exopolysaccharide and metal-doped bioactive glass nanoparticles-based electrospun membranes for chronic wound healing

Abstract

Healing of chronic wounds requires interactive dressings that not only meet basic biological criteria, of biocompatibility, but also offer additional functionalities such as antioxidant and antibacterial properties. In this study, three novel multifunctional nanofibrous membranes based on EPS, an α-glucan exopolysaccharide produced by Lacticaseibacillus rhamnosus P14, blended with PEO as a co-polymer were successfully developed using electrospinning. The membranes incorporated Cu or Ag-doped bioactive glass (BG) nanoparticles, to enhance their functionalities. The structural and thermal properties of the electrospun membranes were characterized using FT-IR, TGA, and DSC analysis. In addition, the surface morphology, fiber size, and porosity were examined by SEM analysis. Additionally, their biological properties, including antioxidant and antibacterial activities, were thoroughly investigated. SEM confirmed the effective electrospinning of the EPS-PEO and EPS-PEO-BG blends from aqueous solutions using optimized process parameters, resulting in the successful incorporation of the BG nanoparticles and uniform fibers with average diameter ranging from 270 to 352 nm. Moreover, DPPH RSA and FRAP assays showed a significant antioxidant capacity for all prepared membranes which is attributed to the EPS component. Moreover, the antibacterial activity revealed a notable inhibition against E. coli and S. aureus after 24 h exposure to the composite membranes. This work presents a novel synergistic approach to developing multifunctional wound dressing by combining the antioxidant properties of EPS, the antibacterial activity of ion-doped bioactive glass nanoparticles, and structural benefits of electrospinning.