Impact of Non-SMC Condensin I Complex Subunit D2 Upregulation on Oral Squamous Cell Carcinoma Prognosis

IF 3.2 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

International dental journal Pub Date : 2025-04-16 DOI:10.1016/j.identj.2024.03.015

Qingying Cui , Shuai Fu , Diping Yu , Ming Li , Yong Li

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引用次数: 0

Abstract

Objective

To explore the influence of non-SMC condensin I complex subunit D2 (NCAPD2) on the prognosis of oral squamous cell carcinoma (OSCC) and the correlation between NCAPD2 and OSCC.

Methods

In this study, NCAPD2 gene expression profiles of OSCC and normal tissues were collected from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). The real-time quantitative polymerase chain reaction (RT-qPCR) was employed to preliminarily validate OSCC cell strains and normal epithelial cell strains. Besides EdU, cell scratch, and transwell assays were performed to assess the proliferation, migration, and invasion of OSCC cell strains with the silence of NCAPD2. Moreover, immunohistochemistry (IHC) staining was utilised to measure the expression of NCAPD2 and tumour-related markers in 74 OSCC specimens. Finally, the Kaplan-Meier analysis was performed to evaluate the influence of NCAPD2 in the prognosis of OSCC.

Results

The expression of NCAPD2 in OSCC tissues was higher than that in normal tissues. Inhibiting NCAPD2 can reduce the proliferation and migration of OSCC cell lines and inhibit the invasion of these cells. The IHC staining results indicated that the high expression of NCAPD2 in OSCC tissues was positively correlated with T stages, Ki67 expression, and affected sites. The Kaplan-Meier analysis results validated that the up-regulated expression of NCAPD2 was significantly correlated with the poor overall survival (OS) of OSCC patients.

Conclusion

NCAPD2 is a potential molecular marker for the poor prognosis of OSCC, and it is expected to become a target for the treatment of this carcinoma.

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非smc凝血素I复合体D2亚基上调对口腔鳞状细胞癌预后的影响

目的探讨非smc凝血素I复合体亚基D2 （NCAPD2）对口腔鳞状细胞癌（OSCC）预后的影响及其与OSCC的相关性。方法从肿瘤基因组图谱（Cancer Genome Atlas， TCGA）和基因表达图谱（gene expression Omnibus， GEO）中收集OSCC和正常组织的NCAPD2基因表达谱。采用实时定量聚合酶链反应（RT-qPCR）对OSCC细胞株和正常上皮细胞株进行初步验证。除EdU外，我们还通过细胞划痕和transwell实验来评估NCAPD2沉默作用下OSCC细胞株的增殖、迁移和侵袭情况。此外，利用免疫组织化学（IHC）染色检测74例OSCC标本中NCAPD2和肿瘤相关标志物的表达。最后通过Kaplan-Meier分析评价NCAPD2对OSCC预后的影响。结果NCAPD2在OSCC组织中的表达明显高于正常组织。抑制NCAPD2可以减少OSCC细胞系的增殖和迁移，抑制这些细胞的侵袭。免疫组化染色结果显示，NCAPD2在OSCC组织中的高表达与T分期、Ki67表达、发病部位呈正相关。Kaplan-Meier分析结果证实，NCAPD2表达上调与OSCC患者总生存期（OS）较差显著相关。结论ncapd2是OSCC不良预后的潜在分子标志物，有望成为OSCC治疗的靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International dental journal 医学-牙科与口腔外科

CiteScore

4.80

自引率

6.10%

发文量

159

审稿时长

63 days

期刊介绍： The International Dental Journal features peer-reviewed, scientific articles relevant to international oral health issues, as well as practical, informative articles aimed at clinicians.