Molecular characterization and functional analysis of Collectin-11 from Hexagrammos otakii

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2025-04-14 DOI:10.1016/j.fsi.2025.110338

Haidong Zha , Haoyue Zhang , Jinmiao Zhong , Yujiang Wang , Liyuan Liu , Shanshan Yu , Yingying Liu

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Abstract

Hexagrammos otakii is an essential economic fish for some coastal cities, but severe and frequent infections have appeared in aquaculture. Previous studies have shown that collectin-11 (CL-11) is a typical soluble pattern recognition receptor capable of recognizing pathogens and initiating the complement cascade by interacting with mannan-binding lectin-associated serine protease-1 (MASP1). Hence, to better understand the functions of CL-11 in Hexagrammos otakii (HoCL-11), we conducted qRT-PCR, LPS and PGN binding, bacterial agglutination, ELISA, and FAR-WESTERN after recombinant expression. As a result, qRT-PCR revealed that the HoCL-11 gene was predominantly expressed in the liver, skin, and ovary tissues of Hexagrammos otakii. Bacterial agglutination and binding assays showed that the recombinant CL-11 protein could recognize various pathogens. ELISA and far-western experiments confirmed that HoCL-11 could bind to MASP1 and MASP2 and may further activate the complement. In conclusion, HoCL-11 is pivotal in teleost Hexagrammos otakii's immune defense, and this study has theoretically supplemented its innate immune mechanism.

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.