Characterization and functional analysis of CsTLR13M/13S, two novel teleost toll-like receptor 13 in antimicrobial immunity

IF 3.9 1区农林科学 Q1 FISHERIES

Aquaculture Pub Date : 2025-04-09 DOI:10.1016/j.aquaculture.2025.742523

Ji-xing Feng, Sheng-jia Wang, Hong-ye Wang, Xue-peng Li

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引用次数: 0

Abstract

In mammals, toll-like receptors 13 (TLR13), as an intracellular endosomal TLR, recognizes various pathogenic microorganism, playing vital roles in the first line of host immunity through myeloid differentiation factor 88 (MyD88) signaling pathway. However, the anti-microbial effect of teleost TLR13 is largely obscure. Here, we reported the expression patterns, signaling pathway and immune property of tongue sole (Cynoglossus semilaevis) TLR13 (CsTLR13). The membrane form of CsTLR13 (CsTLR13M) contains three domain, i.e. extracellular domain (ECD), cytoplasmic TIR domain and transmembrane (TM) domain; the soluble form of CsTLR13 (CsTLR13S) only contains single ECD. CsTLR13M/CsTLR13S share 77.5 % identity with each other, with the same 19 LRRs in the ECD. CsTLR13M/CsTLR13S were constitutively existed in different tissues with similar expression patterns, and regulated by bacterial and viral infection. Recombinant CsTLR13S (rCsTLR13S) could bind to bacteria, with the highest affinity to P. fluorescens, and inhibited the growth of bacteria. In vivo, knockdown of CsTLR13M/CsTLR13S markedly enhanced bacterial infection. Consistently, CsTLR13M/CsTLR13S-deficient peripheral blood leukocytes (PBLs) revealed reduced expression of pro-inflammatory genes, MyD88-signaling related genes, and ROS production, which in line with rCsTLR13S activated the expression of pro-inflammatory and MyD88-signaling related genes. Overall, our findings give new insights into the signaling pathway and function of CsTLR13 in teleost immunity.

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CsTLR13M/13S--两种新型远洋鱼类收费样受体 13--在抗微生物免疫中的特性和功能分析

在哺乳动物中，toll样受体13 （toll-like receptors 13, TLR13）作为细胞内内体TLR，可识别多种病原微生物，通过髓样分化因子88 （myeloid differentiation factor 88, MyD88）信号通路在宿主免疫第一线发挥重要作用。然而，硬骨鱼TLR13的抑菌作用在很大程度上是未知的。本文报道了舌鳎（Cynoglossus semilaevis） TLR13 （CsTLR13）的表达模式、信号通路和免疫特性。CsTLR13 （CsTLR13M）的膜形态包含三个结构域，即胞外结构域（ECD）、胞质TIR结构域和跨膜结构域（TM）；CsTLR13的可溶性形式（CsTLR13S）仅含有单个ECD。CsTLR13M/CsTLR13S在ECD中具有相同的19个lrr，彼此具有77.5%的同源性。CsTLR13M/CsTLR13S组成型存在于不同组织中，表达模式相似，受细菌和病毒感染的调控。重组CsTLR13S （rCsTLR13S）能与细菌结合，对P. fluorescens的亲和力最高，能抑制细菌的生长。在体内，敲低CsTLR13M/CsTLR13S显著增强细菌感染。同样，CsTLR13M/ cstlr13s缺陷外周血白细胞（PBLs）显示促炎基因、myd88信号相关基因的表达减少，ROS的产生减少，这与rCsTLR13S一致，激活了促炎基因和myd88信号相关基因的表达。总之，我们的研究结果对CsTLR13在硬骨鱼免疫中的信号通路和功能有了新的认识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture 农林科学-海洋与淡水生物学

CiteScore

8.60

自引率

17.80%

发文量

1246

审稿时长

56 days

期刊介绍： Aquaculture is an international journal for the exploration, improvement and management of all freshwater and marine food resources. It publishes novel and innovative research of world-wide interest on farming of aquatic organisms, which includes finfish, mollusks, crustaceans and aquatic plants for human consumption. Research on ornamentals is not a focus of the Journal. Aquaculture only publishes papers with a clear relevance to improving aquaculture practices or a potential application.