Functionally distinct GABAergic amacrine cell types regulate spatiotemporal encoding in the mouse retina

IF 21.2 1区 医学 Q1 NEUROSCIENCES
Akihiro Matsumoto, Jacqueline Morris, Loren L. Looger, Keisuke Yonehara
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Abstract

GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the mammalian central nervous system. GABAergic neuronal types play important roles in neural processing and the etiology of neurological disorders; however, there is no comprehensive understanding of their functional diversity. Here we perform two-photon imaging of GABA release in the inner plexiform layer of male and female mice retinae (8–16 weeks old) using the GABA sensor iGABASnFR2. By applying varied light stimuli to isolated retinae, we reveal over 40 different GABA-releasing neuron types. Individual types show layer-specific visual encoding within inner plexiform layer sublayers. Synaptic input and output sites are aligned along specific retinal orientations. The combination of cell type-specific spatial structure and unique release kinetics enables inhibitory neurons to sculpt excitatory signals in response to a wide range of behaviorally relevant motion structures. Our findings emphasize the importance of functional diversity and intricate specialization of GABAergic neurons in the central nervous system.

Abstract Image

功能上不同的gaba能无突细胞类型调节小鼠视网膜的时空编码
GABA (γ-氨基丁酸)是哺乳动物中枢神经系统的主要抑制性神经递质。gaba能神经元类型在神经加工和神经系统疾病的病因学中起重要作用;然而,对其功能多样性的认识尚不全面。本研究使用GABA传感器iGABASnFR2对8-16周龄雄性和雌性小鼠视网膜内丛状层GABA释放进行双光子成像。通过对离体视网膜施加不同的光刺激,我们发现了40多种不同的gaba释放神经元类型。单个类型在内网状层子层中显示特定于层的视觉编码。突触输入和输出位点沿着特定的视网膜方向排列。细胞类型特异性的空间结构和独特的释放动力学相结合,使抑制性神经元能够响应广泛的行为相关运动结构来塑造兴奋信号。我们的研究结果强调了中枢神经系统中gaba能神经元功能多样性和复杂特化的重要性。
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来源期刊
Nature neuroscience
Nature neuroscience 医学-神经科学
CiteScore
38.60
自引率
1.20%
发文量
212
审稿时长
1 months
期刊介绍: Nature Neuroscience, a multidisciplinary journal, publishes papers of the utmost quality and significance across all realms of neuroscience. The editors welcome contributions spanning molecular, cellular, systems, and cognitive neuroscience, along with psychophysics, computational modeling, and nervous system disorders. While no area is off-limits, studies offering fundamental insights into nervous system function receive priority. The journal offers high visibility to both readers and authors, fostering interdisciplinary communication and accessibility to a broad audience. It maintains high standards of copy editing and production, rigorous peer review, rapid publication, and operates independently from academic societies and other vested interests. In addition to primary research, Nature Neuroscience features news and views, reviews, editorials, commentaries, perspectives, book reviews, and correspondence, aiming to serve as the voice of the global neuroscience community.
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