Exploring the chemical behaviors of dissolved organic matter to thermal hydrolysis temperature at the molecular level and its fate in anaerobic membrane bioreactor

IF 11.4 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Water Research Pub Date : 2025-04-15 DOI:10.1016/j.watres.2025.123650

Jian Yin , Teng Cai , Yizhi Zhang , Qicai Dai , Yijing Gao , Siqin Li , Xueqin Lu , Guangyin Zhen

{"title":"Exploring the chemical behaviors of dissolved organic matter to thermal hydrolysis temperature at the molecular level and its fate in anaerobic membrane bioreactor","authors":"Jian Yin , Teng Cai , Yizhi Zhang , Qicai Dai , Yijing Gao , Siqin Li , Xueqin Lu , Guangyin Zhen","doi":"10.1016/j.watres.2025.123650","DOIUrl":null,"url":null,"abstract":"<div><div>Thermal hydrolysis pretreatment (THP) coupled with anaerobic membrane bioreactor (AnMBR) to enhance biomass bioconversion and methane production is a promising biotechnology. Herein, we shed light on the effects of THP temperature on molecular structure changes of dissolved organic matter of sewage sludge and food waste and its underlying mechanisms on hydrolysis, and methane bioconversion. The optimal THP condition was 160 °C, with a 1.87-times increase in soluble chemical oxygen demand (6.35 ± 0.09 g/L). FT-ICR MS indicated most of the compounds were biodegradable after 160 °C THP treatment, which had low aromatic or polarity, corresponding to protein/amino sugars and unsaturated hydrocarbon regions. Side reactions, like Maillard reaction and caramelization, induced the production of recalcitrant formulas with high hydrophobic and aromatic structure content (lower O/C and H/C values). These recalcitrant formulas attributed to carboxylic-rich alicyclic molecules (CRAM) exhibited poor biodegradability. For homologous DOMs sharing the same Kendrick mass defect (KMD), compounds exhibiting lower nominal oxidation state of carbon (NOSC), higher H/C ratios, and lower O/C ratios tend to exhibit greater biodegradability. Microbial analysis revealed that samples after THP pretreatment showed enhanced enrichment of both organic matter-degrading bacteria (e.g., Prolixibacteraceae, Anaerolineae and SJA-15) and methanogenic archaea (e.g., Methanosaeta, Methanobacterium, and Candidatus Methanofastidiosum) during the AD process. leading to a synergistic effect among microorganisms (such as <em>Anaerolineae</em> and <em>Methanosaeta</em>). Our findings highlight the interactive mechanism among molecular-level DOMs composition, microbial community succession, and AnMBR's performance, which provides a basis for an in-depth understanding of the THP strategy on anaerobic digestion.</div></div>","PeriodicalId":443,"journal":{"name":"Water Research","volume":"281 ","pages":"Article 123650"},"PeriodicalIF":11.4000,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Water Research","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0043135425005603","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}

引用次数: 0

Abstract

Thermal hydrolysis pretreatment (THP) coupled with anaerobic membrane bioreactor (AnMBR) to enhance biomass bioconversion and methane production is a promising biotechnology. Herein, we shed light on the effects of THP temperature on molecular structure changes of dissolved organic matter of sewage sludge and food waste and its underlying mechanisms on hydrolysis, and methane bioconversion. The optimal THP condition was 160 °C, with a 1.87-times increase in soluble chemical oxygen demand (6.35 ± 0.09 g/L). FT-ICR MS indicated most of the compounds were biodegradable after 160 °C THP treatment, which had low aromatic or polarity, corresponding to protein/amino sugars and unsaturated hydrocarbon regions. Side reactions, like Maillard reaction and caramelization, induced the production of recalcitrant formulas with high hydrophobic and aromatic structure content (lower O/C and H/C values). These recalcitrant formulas attributed to carboxylic-rich alicyclic molecules (CRAM) exhibited poor biodegradability. For homologous DOMs sharing the same Kendrick mass defect (KMD), compounds exhibiting lower nominal oxidation state of carbon (NOSC), higher H/C ratios, and lower O/C ratios tend to exhibit greater biodegradability. Microbial analysis revealed that samples after THP pretreatment showed enhanced enrichment of both organic matter-degrading bacteria (e.g., Prolixibacteraceae, Anaerolineae and SJA-15) and methanogenic archaea (e.g., Methanosaeta, Methanobacterium, and Candidatus Methanofastidiosum) during the AD process. leading to a synergistic effect among microorganisms (such as Anaerolineae and Methanosaeta). Our findings highlight the interactive mechanism among molecular-level DOMs composition, microbial community succession, and AnMBR's performance, which provides a basis for an in-depth understanding of the THP strategy on anaerobic digestion.

Abstract Image

查看原文本刊更多论文

在分子水平上探讨溶解有机物对热水解温度的化学行为及其在厌氧膜生物反应器中的归宿

热水解预处理（THP）联合厌氧膜生物反应器（AnMBR）提高生物质转化和甲烷产量是一种很有前景的生物技术。本研究揭示了THP温度对污水污泥和食物垃圾中溶解有机物分子结构变化的影响及其对水解和甲烷生物转化的潜在机制。最佳THP条件为160℃，可使可溶性化学需氧量增加1.87倍（6.35±0.09 g/L）。FT-ICR MS表明，经160°C THP处理后，大部分化合物具有低芳香族或极性，对应于蛋白质/氨基糖和不饱和烃区。副反应，如美拉德反应和焦糖化反应，诱导生成具有高疏水和芳香结构含量（较低的O/C和H/C值）的顽固性配方。这些顽固性配方归因于富羧基脂环分子（CRAM），表现出较差的生物降解性。对于具有相同Kendrick质量缺陷（KMD）的同源dom，具有较低标称碳氧化态（NOSC）、较高H/C比和较低O/C比的化合物往往具有较好的生物可降解性。微生物分析显示，THP预处理后的样品在AD过程中，有机物降解细菌（如Prolixibacteraceae、Anaerolineae和SJA-15）和产甲烷古细菌（如Methanosaeta、Methanobacterium和Candidatus Methanofastidiosum）的富集都有所增加。导致微生物（如厌氧菌和甲烷菌）之间的协同作用。我们的研究结果强调了分子水平上DOMs组成、微生物群落演替和AnMBR性能之间的相互作用机制，为深入了解THP对厌氧消化的策略提供了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Water Research 环境科学-工程：环境

CiteScore

20.80

自引率

9.40%

发文量

1307

审稿时长

38 days

期刊介绍： Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include: •Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management; •Urban hydrology including sewer systems, stormwater management, and green infrastructure; •Drinking water treatment and distribution; •Potable and non-potable water reuse; •Sanitation, public health, and risk assessment; •Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions; •Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment; •Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution; •Environmental restoration, linked to surface water, groundwater and groundwater remediation; •Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts; •Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle; •Socio-economic, policy, and regulations studies.