Enhanced apoptosis in damaged laminar tissue of acute laminitis induced by oligofructose overload in dairy cows

IF 1.4 3区农林科学 Q4 IMMUNOLOGY

Veterinary immunology and immunopathology Pub Date : 2025-04-08 DOI:10.1016/j.vetimm.2025.110935

Muhammad Abid Hayat , Jiafeng Ding , Xianhao Zhang , Tao Liu , Jiantao Zhang , Hong Bin Wang

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Abstract

Dairy cow laminitis leads to massive financial losses and animal health issues in the worldwide dairy sector. Apoptosis may be an important factor in the epidermal attachment failure. This study explored the laminar tissue apoptotic-related gene and protein status with oligofructose (OF)-induced laminitis in dairy cows. Twelve clinically healthy, non-pregnant Chinese Holstein cows were randomly divided into two groups of six cows each: the control group and the oligofructose overload group (OF group), respectively. At 0 h, 17 g/kg BW of OF dissolved in 20 mL/kg BW of warm deionized water was gavaged to the OF dairy cows through a stomach tube, while the control cows were given the same dose of deionized water in the same way. After 72 h, laminar tissue samples in both groups were collected to express genes and proteins. Compared with the control cows, the gene expression of Bcl2 significantly reduced in the OF cows laminar tissue. The gene expression of Bax and P53 significantly enhanced in the laminar tissue of OF cows compared to the control cows. The expression of Bcl2 protein significantly decreased, whereas the expression of Bax and Bif1, caspase3, caspase8, and caspase9/9p proteins significantly increased in the OF cows' laminar tissues than in the control cows. However, the distribution of Bax and P53 proteins significantly enhanced in the OF cows' laminar tissues relative to the control cows. In conclusion, imbalanced gene and protein status may represent the primary cause of the epidermal attachment failure, which confirmed the increased apoptosis in laminar tissue of sick cows.

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低聚果糖过量诱导奶牛急性板层炎损伤层组织凋亡增加

奶牛板膜炎在世界范围内导致巨大的经济损失和动物健康问题。细胞凋亡可能是表皮附着失败的重要因素。本研究旨在探讨低聚果糖（OF）诱导的奶牛板层组织凋亡相关基因及蛋白的表达情况。选取12头临床健康、未怀孕的中国荷斯坦奶牛，随机分为2组，每组6头，分别为对照组和低聚果糖过量组（of组）。在0 h时，将溶解于20 mL/kg BW的温去离子水中17 g/kg BW的of通过胃管灌胃，对照组奶牛以相同的方式给予相同剂量的去离子水。72 h后，收集两组层流组织样品表达基因和蛋白。与对照奶牛相比，Bcl2基因在of奶牛层流组织中的表达显著降低。与对照奶牛相比，of奶牛层流组织中Bax和P53基因的表达显著增强。Bcl2蛋白的表达量显著降低，Bax、Bif1、caspase3、caspase8和caspase9/9p蛋白的表达量显著高于对照组。然而，与对照奶牛相比，Bax和P53蛋白在of奶牛层流组织中的分布显著增强。综上所述，基因和蛋白的失衡可能是导致表皮附着失败的主要原因，这证实了病牛层状组织中细胞凋亡的增加。

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来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.