P2 component latency of fVEP as a bioindicator for clinical and diagnostic use in visual pathologies

IF 3 2区医学 Q1 OPHTHALMOLOGY

Experimental eye research Pub Date : 2025-04-08 DOI:10.1016/j.exer.2025.110381

Fei Liao , Pedro de la Villa , Haitao Liu , Francisco Germain , Ting Wang

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引用次数: 0

Abstract

Purpose

The signaling of flash visual evoked potential (fVEP) derives from the retina, but how retinal activity influences fVEP remains unclear. This work aimed to decipher the specific retinal kinetic contributions to fVEP response.

Methods

Monocular and simultaneous recordings of flash VEP and electroretinogram were performed. Healthy and adult mice C57BL/6J were used. The right eye was injected intravitreally with 1 μL of PBS containing 25 mM APB, 10 mM Bicuculline, 30 mM DNQX, 100 mM Glutamate, 100 mM GABA, 5 mM TPMPA, or 25 mM HEPES. The left eye was injected with 1 μL of PBS and then wore an opaque patch. The amplitude and latency of fVEP were analyzed in detail.

Results

In the control group, at light intensity ≤ 0.1 cd·s/m², four robust components of the fVEP recordings, N1, P1, N2, and P2, were identified in dark adaptation conditions. After administration reagents, N1 and P1 components were abolished by APB, Bicuculline, DNQX or TPMPA, but were preserved by GABA/Glutamate or HEPES. Notably, N2 and P2 components were always kept. The latency and amplitude of fVEP were shown to be stimulus-dependent. Nevertheless, the amplitude showed greater inter-individual variability than latency.

Conclusion

N1 and P1 components are strongly related to rod photoreceptor activity and/or the level of horizontal cell excitation. Latency, rather than fVEP amplitude, could be a good biomarker for clinical and diagnostic purposes, particularly the P2 latency in the rod-driven scotopic response.

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fVEP P2组分潜伏期作为临床和视觉病理诊断的生物指标

目的闪烁视觉诱发电位（fVEP）信号来源于视网膜，但视网膜活动如何影响fVEP尚不清楚。这项工作旨在破译特定的视网膜动力学贡献的fVEP反应。方法单目和同步记录VEP和视网膜电图。采用健康小鼠和成年小鼠C57BL/6J。右眼玻璃体内注射1 μL PBS，其中含有25 mM APB、10 mM Bicuculline、30 mM DNQX、100 mM谷氨酸、100 mM GABA、5 mM TPMPA或25 mM HEPES。左眼注射1 μL PBS，佩戴不透明眼罩。详细分析了fVEP的幅值和潜伏期。结果对照组在光强≤0.1 cd·s/m2条件下，在暗适应条件下，fVEP记录的4个稳健分量分别为N1、P1、N2和P2。给药后，APB、Bicuculline、DNQX或TPMPA使N1和P1成分消失，而GABA/Glutamate或HEPES保留了N1和P1成分。值得注意的是，N2和P2组分一直保持不变。fVEP的潜伏期和振幅是刺激依赖性的。然而，振幅表现出比潜伏期更大的个体间变异。结论n1和P1组分与视杆光感受器活性和/或水平细胞兴奋水平密切相关。潜伏期，而不是fVEP振幅，可能是临床和诊断目的的良好生物标志物，特别是P2潜伏期在棒驱动的暗斑反应中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental eye research 医学-眼科学

CiteScore

6.80

自引率

5.90%

发文量

323

审稿时长

66 days

期刊介绍： The primary goal of Experimental Eye Research is to publish original research papers on all aspects of experimental biology of the eye and ocular tissues that seek to define the mechanisms of normal function and/or disease. Studies of ocular tissues that encompass the disciplines of cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology or microbiology are most welcomed. Manuscripts that are purely clinical or in a surgical area of ophthalmology are not appropriate for submission to Experimental Eye Research and if received will be returned without review.