T2T genome, pan-genome analysis, and heat stress response genes in Rhododendron species

IF 23.7 Q1 MICROBIOLOGY
iMeta Pub Date : 2025-03-05 DOI:10.1002/imt2.70010
Xiaojing Wang, Ping Zhou, Xiaoyu Hu, Yun Bai, Chenhao Zhang, Yanhong Fu, Ruirui Huang, Niu Suzhen, Xiaoming Song
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引用次数: 0

Abstract

This study reports the first high-quality telomere-to-telomere (T2T) Rhododendron liliiflorum genome with 11 chromosomes that are gap free. The 24 telomeres and all 13 centromeres detected in this genome, which reached the highest quality gold level. In addition, other three Rhododendron species were sequenced and assembled to the chromosomal level. Based on 15 Rhododendron genomes, we conducted a pan-genome analysis of genus Rhododendron. Combining the genome and whole transcriptome sequencing, we identified several key genes and miRNAs related to the heat stress, which were further verified by transgenic experiments. Our findings provide rich resources for comparative and functional genomics studies of Rhododendron species.

Abstract Image

杜鹃花T2T基因组、泛基因组分析及热应激反应基因
本研究报道了第一个具有11条无间隙染色体的高质量的端粒到端粒(T2T)百合杜鹃基因组。在该基因组中检测到的24个端粒和全部13个着丝粒,达到了最高质量的黄金水平。此外,对另外3种杜鹃花进行了染色体水平的测序和组装。基于15个杜鹃花基因组,对杜鹃花属植物进行了泛基因组分析。结合基因组和全转录组测序,我们鉴定出与热胁迫相关的几个关键基因和mirna,并通过转基因实验进一步验证。研究结果为杜鹃属植物的比较和功能基因组学研究提供了丰富的资源。
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CiteScore
10.80
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