A novel phosvitin phosphopeptide (Glu-Asp-Asp-pSer-pSer) as a potential food ingredient to intervene in osteoporosis: A potential mechanism study on promoting the osteogenic differentiation of MC3T3-E1 cells

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods Pub Date : 2025-04-14 DOI:10.1016/j.jff.2025.106821

Tianrui Xue , Tingting Gong , Wei Zhao, Mingran Zhang, Binfei Lv, Huiping Liu, Qi Xu, Xiaowei Zhang

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Abstract

Food-derived peptides with strong calcium-binding and osteogenic activity are promising functional ingredients for osteoporosis intervention. This study aims to explore whether a novel phosvitin phosphopeptide (Glu-Asp-Asp-pSer-pSer, EDDpSpS) with excellent calcium binding ability can promote the osteogenic differentiation of MC3T3-E1 cells and its potential mechanism. Network pharmacology analysis of EDDpSpS regulating osteoblast differentiation identified that the focal adhesion was a key signaling pathway, which is closely related to cell differentiation. Molecular simulations demonstrated that EDDpSpS was strongly bound to the target proteins within the focal adhesion signaling pathway and maintains a stable state. In addition, EDDpSpS had the potential to activate the key target protein Src tyrosine kinase (Src). Results of cell experiments showed that EDDpSpS (> 100 μg/mL) significantly promoted the differentiation and mineralization of MC3T3-E1 cells. Transcriptome analysis verified that the expression of paxillin (PXN), Src, actin beta/gamma 1 (ACTG1), and other osteoblast genes were regulated by EDDpSpS, leading to an up-regulation of the focal adhesion. In this study, the effect and the potential mechanism of EDDpSpS on osteoblast differentiation were clarified. EDDpSpS may be used as an effective dual-effect food ingredient for developing functional foods targeting osteoporosis.

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一种新型磷脂酰磷酸肽（Glu-Asp-Asp-pSer-pSer）作为干预骨质疏松症的潜在食品成分：促进 MC3T3-E1 细胞成骨分化的潜在机制研究

食源性肽具有很强的钙结合和成骨活性，是治疗骨质疏松症的重要功能成分。本研究旨在探讨一种具有优异钙结合能力的新型磷维素磷酸肽（Glu-Asp-Asp-pSer-pSer, EDDpSpS）是否能促进MC3T3-E1细胞的成骨分化及其潜在机制。EDDpSpS调控成骨细胞分化的网络药理学分析发现，局灶黏附是调控成骨细胞分化的关键信号通路。分子模拟表明，EDDpSpS与黏附信号通路内的靶蛋白紧密结合，并保持稳定状态。此外，EDDpSpS具有激活关键靶蛋白Src酪氨酸激酶（Src）的潜力。细胞实验结果表明，EDDpSpS (>；100 μg/mL)显著促进MC3T3-E1细胞分化和矿化。转录组分析证实，paxillin （PXN）、Src、actin β / γ 1 （ACTG1）等成骨细胞基因的表达受EDDpSpS调控，导致局点黏附上调。本研究阐明了EDDpSpS对成骨细胞分化的影响及其潜在机制。EDDpSpS可作为一种有效的双效食品成分，用于开发针对骨质疏松症的功能性食品。

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来源期刊

Journal of Functional Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

9.60

自引率

1.80%

发文量

428

审稿时长

76 days

期刊介绍： Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies. The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients. The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.