Copper Nanoparticle Decorated Multilayer Nanocoatings for Controlled Nitric Oxide Release and Antimicrobial Performance with Biosafety

IF 5.5 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomacromolecules Pub Date : 2025-03-31 DOI:10.1021/acs.biomac.4c0179810.1021/acs.biomac.4c01798

Hyejoong Jeong*, Jiwoong Heo, Moonhyun Choi and Jinkee Hong*,

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Abstract

Biomedical device-related bacterial infections are a leading cause of mortality, and traditional antibiotics contribute to resistance. Various surface modification strategies have been explored, but effective clinical solutions remain limited. This study introduces a novel antibacterial nanocoating with copper nanoparticles (CuNPs) that triggers localized nitric oxide (NO) release. The multilayered nanocoating is created using branched polyethylenimine (BPEI) and poly(acrylic acid) (PAA) via a Layer-by-Layer assembly method. CuNP-decorated nanocoatings are formed by reducing copper ions coordinated with amine/carboxylic acid groups. In a physiological environment, CuNPs oxidize to Cu(I), promoting NO release from endogenous NO donors. The nanocoating’s thickness is adjustable to regulate amount of CuNPs and NO flux. The optimal thickness for effective NO release against Staphylococcus aureus and Pseudomonas aeruginosa is identified, preventing microbial adhesion and biofilm formation. Importantly, the coating remains cytocompatible due to minimal CuNPs, physiological NO levels, and stable coating properties under physiological conditions.

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与生物医学设备相关的细菌感染是导致死亡的主要原因，而传统抗生素会导致耐药性的产生。人们探索了各种表面改性策略，但有效的临床解决方案仍然有限。本研究介绍了一种新型抗菌纳米涂层，该涂层含有纳米铜粒子（CuNPs），可触发局部一氧化氮（NO）释放。这种多层纳米涂层是使用支化聚乙烯亚胺（BPEI）和聚丙烯酸（PAA）通过逐层组装法制成的。CuNP装饰纳米涂层是由与胺/羧酸基团配位的还原铜离子形成的。在生理环境中，CuNPs 会氧化成 Cu(I)，促进内源性 NO 供体释放 NO。纳米涂层的厚度可调，以调节 CuNPs 的数量和 NO 的通量。已确定了能有效抑制金黄色葡萄球菌和铜绿假单胞菌释放 NO 的最佳厚度，从而防止微生物粘附和生物膜的形成。重要的是，由于 CuNPs 含量极低、NO 含量达到生理水平，而且涂层在生理条件下具有稳定的特性，因此涂层仍具有细胞兼容性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomacromolecules 化学-高分子科学

CiteScore

10.60

自引率

4.80%

发文量

417

审稿时长

1.6 months

期刊介绍： Biomacromolecules is a leading forum for the dissemination of cutting-edge research at the interface of polymer science and biology. Submissions to Biomacromolecules should contain strong elements of innovation in terms of macromolecular design, synthesis and characterization, or in the application of polymer materials to biology and medicine. Topics covered by Biomacromolecules include, but are not exclusively limited to: sustainable polymers, polymers based on natural and renewable resources, degradable polymers, polymer conjugates, polymeric drugs, polymers in biocatalysis, biomacromolecular assembly, biomimetic polymers, polymer-biomineral hybrids, biomimetic-polymer processing, polymer recycling, bioactive polymer surfaces, original polymer design for biomedical applications such as immunotherapy, drug delivery, gene delivery, antimicrobial applications, diagnostic imaging and biosensing, polymers in tissue engineering and regenerative medicine, polymeric scaffolds and hydrogels for cell culture and delivery.