Phytochemical characterization, antioxidant, enzyme inhibitory and anti-inflammatory activities of Hedychium coccineum Buch.-Ham. ex Sm. rhizome

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology Pub Date : 2025-04-12 DOI:10.1016/j.bse.2025.105022

Yuanquan Ran , Dan Xie , Dan Long , Lu Jin , Guo Chen , Bing Yang , Dongxin Tang , Minyi Tian

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引用次数: 0

Abstract

Hedychium coccineum Buch.-Ham. ex Sm. is a highly valuable plant with ornamental, edible, and medicinal properties. However, there are few studies on its phytochemicals and biological activities. Therefore, this study focused on phytochemical analyses of H. coccineum rhizome water extract (WE) and 70 % ethanol extract (EE) and their antioxidant, enzyme inhibitory, and anti-inflammatory properties. Chemical composition analysis indicated H. coccineum rhizome WE and EE had high total phenolic (37.33–42.45 mg GAEs/g extract) and flavonoid (33.79–212.06 mg REs/g extract) contents and further UHPLC-Q-Orbitrap-MS analysis identified 75 compounds, including 14 phenolic compounds and 23 flavonoids. In the DPPH and ABTS radical scavenging tests, WE (29.88 ± 1.41 μg/mL and 26.60 ± 0.70 μg/mL, respectively) and EE (18.15 ± 0.51 μg/mL and 11.73 ± 0.30 μg/mL, respectively) displayed superior antioxidant effects than the positive control BHT (76.07 ± 8.40 μg/mL and 49.85 ± 4.46 μg/mL, respectively). Except for the weak inhibition on tyrosinase and cholinesterases, it is interesting that the α-glucosidase inhibitory capacities of WE (IC₅₀: 22.87 ± 2.17 μg/mL) and EE (IC₅₀: 5.41 ± 0.44 μg/mL) were superior to that of acarbose (IC₅₀: 226.69 ± 16.14 μg/mL). Additionally, H. coccineum rhizome WE and EE at non-toxic doses significantly suppressed the LPS-induced secretion of pro-inflammatory mediators (NO) and cytokines (TNF-α and IL-6) in RAW264.7 cells. Hence, H. coccineum rhizome can be a potential source of active natural compounds for application in functional foods and pharmaceuticals.

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红草的植物化学特性、抗氧化、酶抑制和抗炎活性。Sm。粉末

尾骨草-火腿。Sm。是一种具有观赏、食用和药用价值的高价值植物。然而，对其植物化学成分和生物活性的研究很少。因此，本研究重点分析了鸡尾骨水提取物（WE）和70%乙醇提取物（EE）的植物化学性质及其抗氧化、酶抑制和抗炎性能。化学成分分析表明，鸡尾藤提取物和粗糖提取物中总酚（37.33 ~ 42.45 mg GAEs/g）和类黄酮（33.79 ~ 212.06 mg REs/g）含量较高，UHPLC-Q-Orbitrap-MS分析鉴定出75种化合物，其中酚类化合物14种，类黄酮23种。在DPPH和ABTS自由基清除试验中，WE（分别为29.88±1.41 μg/mL和26.60±0.70 μg/mL）和EE（分别为18.15±0.51 μg/mL和11.73±0.30 μg/mL）的抗氧化作用优于阳性对照BHT（分别为76.07±8.40 μg/mL和49.85±4.46 μg/mL）。除了对酪氨酸酶和胆碱酯酶的抑制作用较弱外，WE （IC50: 22.87±2.17 μg/mL）和EE （IC50: 5.41±0.44 μg/mL）对α-葡萄糖苷酶的抑制能力均优于阿卡波糖（IC50: 226.69±16.14 μg/mL）。此外，无毒剂量的鸡尾骨根WE和EE显著抑制lps诱导的RAW264.7细胞中促炎介质（NO）和细胞因子（TNF-α和IL-6）的分泌。因此，瓢虫根茎可作为活性天然化合物的潜在来源，应用于功能性食品和药品中。

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来源期刊

Biochemical Systematics and Ecology 生物-进化生物学

CiteScore

3.00

自引率

12.50%

发文量

147

审稿时长

43 days

期刊介绍： Biochemical Systematics and Ecology is devoted to the publication of original papers and reviews, both submitted and invited, in two subject areas: I) the application of biochemistry to problems relating to systematic biology of organisms (biochemical systematics); II) the role of biochemistry in interactions between organisms or between an organism and its environment (biochemical ecology). In the Biochemical Systematics subject area, comparative studies of the distribution of (secondary) metabolites within a wider taxon (e.g. genus or family) are welcome. Comparative studies, encompassing multiple accessions of each of the taxa within their distribution are particularly encouraged. Welcome are also studies combining classical chemosystematic studies (such as comparative HPLC-MS or GC-MS investigations) with (macro-) molecular phylogenetic studies. Studies that involve the comparative use of compounds to help differentiate among species such as adulterants or substitutes that illustrate the applied use of chemosystematics are welcome. In contrast, studies solely employing macromolecular phylogenetic techniques (gene sequences, RAPD studies etc.) will be considered out of scope. Discouraged are manuscripts that report known or new compounds from a single source taxon without addressing a systematic hypothesis. Also considered out of scope are studies using outdated and hard to reproduce macromolecular techniques such as RAPDs in combination with standard chemosystematic techniques such as GC-FID and GC-MS.