Influence of altitudinal variation on the chemodiversity and in-vitro antioxidant potential of essential oils of Micromeria biflora (Buch. -Ham. ex D. Don) Benth. from Uttarakhand, India

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology Pub Date : 2025-04-13 DOI:10.1016/j.bse.2025.105019

Manisha Joshi , Geeta Tewari , Chitra Pande , Hemant K. Pandey , Girish C. Kharkwal , Aabha Gangwar , Lalit M. Tewari , Mamta Bisht

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Abstract

Micromeria biflora, a valuable medicinal herb of the family Lamiaceae, is abundantly present in different regions of Uttarakhand, India. The essential oils extracted by hydrodistillation were analysed by GC-FID and GC-MS and 99.6 %, 96.2 %, 97.8 %, and 99.0 % of essential oil were identified in the samples from Pithoragarh (MBP), Champawat (MBC), Nainital (MBN) and Almora (MBA), respectively. The essential oil composition was significantly affected by locations and altitude, as camphor (61.0 %) was dominant in MBP, β-(E)-caryophyllene was dominant in MBC (32.9 %) and MBA (42.6 %), while germacrene D-4-ol (33.4 %) in MBN. Camphor and germacrene-D-4-ol were identified for the first time in this study. The antioxidant potential was measured by DPPH, ABTS scavenging and metal chelation assays. It was observed that the oil yield and IC₅₀ values were inversely proportional to altitude. M. biflora collected at the lowest altitude (MBP) showed the highest oil yield and significant antioxidant properties, making it a promising source of camphor. In-silico molecular docking along with pass prediction and the Lipinski rule were also analysed.

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海拔高度变化对印度北阿坎德邦 Micromeria biflora (Buch. -Ham. ex D. Don) Benth. 精油化学多样性和体外抗氧化潜力的影响

双花小锥草是一种珍贵的兰科草药，在印度北阿坎德邦的不同地区大量存在。采用气相色谱- fid和气相色谱-质谱法对加氢蒸馏法提取的精油进行了分析，在皮托拉嘎尔（MBP）、champaat （MBC）、奈尼塔尔（MBN）和阿尔莫拉（MBA）样品中分别鉴定出99.6%、96.2%、97.8%和99.0%的精油。挥发油成分受地理位置和海拔的影响显著，在MBP中以樟脑（61.0%）为主，在MBC（32.9%）和MBA（42.6%）中以β-（E)-石竹烯(β- E)-石竹烯）为主，在MBN中以germacrene D-4-ol（33.4%）为主。本研究首次鉴定出樟脑和d -4-醇。通过DPPH、ABTS清除和金属螯合实验测定其抗氧化能力。结果表明，产油率和IC50值与海拔高度成反比。在最低海拔采集的双歧藤具有最高的产油率和显著的抗氧化性能，是一种很有前景的樟脑来源。并对分子对接与通度预测和利平斯基规则进行了分析。

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来源期刊

Biochemical Systematics and Ecology 生物-进化生物学

CiteScore

3.00

自引率

12.50%

发文量

147

审稿时长

43 days

期刊介绍： Biochemical Systematics and Ecology is devoted to the publication of original papers and reviews, both submitted and invited, in two subject areas: I) the application of biochemistry to problems relating to systematic biology of organisms (biochemical systematics); II) the role of biochemistry in interactions between organisms or between an organism and its environment (biochemical ecology). In the Biochemical Systematics subject area, comparative studies of the distribution of (secondary) metabolites within a wider taxon (e.g. genus or family) are welcome. Comparative studies, encompassing multiple accessions of each of the taxa within their distribution are particularly encouraged. Welcome are also studies combining classical chemosystematic studies (such as comparative HPLC-MS or GC-MS investigations) with (macro-) molecular phylogenetic studies. Studies that involve the comparative use of compounds to help differentiate among species such as adulterants or substitutes that illustrate the applied use of chemosystematics are welcome. In contrast, studies solely employing macromolecular phylogenetic techniques (gene sequences, RAPD studies etc.) will be considered out of scope. Discouraged are manuscripts that report known or new compounds from a single source taxon without addressing a systematic hypothesis. Also considered out of scope are studies using outdated and hard to reproduce macromolecular techniques such as RAPDs in combination with standard chemosystematic techniques such as GC-FID and GC-MS.