Fibroblasts fluctuating between mesenchyme and epithelium are involved in hair follicle mesenchyme development

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-04-13 DOI:10.1016/j.bbrep.2025.102006

Yoshikazu Hirose , Asaka Miura , Yuya Ouchi , Tomomi Kitayama , Souki Omura , Takashi Shimbo , Akio Tanaka , Manabu Fujimoto , Kotaro Saga , Katsuto Tamai

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引用次数: 0

Abstract

The transition between the mesenchyme and epithelium contributes to the development of various tissues. During skin development, epithelial-mesenchymal transition in the ectodermal epithelia is involved in the development of the dermal mesenchyme in early embryos. However, the precise roles and functions of epithelial-mesenchymal/mesenchymal-epithelial transition in cutaneous development have not been fully elucidated. In this study, we aimed to elucidate these roles and functions in the neonatal mouse skin. We conducted single-cell RNA sequencing and immunohistochemical analyses to search for Pdgfra-expressing (Pα⁺) fibroblasts with transition activities to/from Krt5-expressing keratinocytes. We determined that the Pα⁺/Krt5-lineage (K5^lin+) fibroblasts significantly contributed to developing hair follicle dermal stem cells to generate lower dermal papilla cells and lower dermal sheath cells. In the developing mouse skin, K5^lin⁺ fibroblasts appeared concurrently with hair follicle development and formed outer edge cells in the early dermal papilla on embryonic day 16.5. K5^lin⁺ hair follicle mesenchymal cells were also maintained in aged mouse skin. These results provide insights into the role and function of the transition between the mesenchyme and epithelium in hair follicle development and maintenance.

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间充质和上皮之间的转变有助于各种组织的发育。在皮肤发育过程中，外胚层上皮的上皮-间充质转变参与了早期胚胎真皮间充质的发育。然而，上皮-间质/间质-上皮转化在皮肤发育中的确切作用和功能尚未完全阐明。本研究旨在阐明新生小鼠皮肤的这些作用和功能。我们进行了单细胞 RNA 测序和免疫组化分析，寻找表达 Pdgfra（Pα+）的成纤维细胞与表达 Krt5 的角质形成细胞之间的过渡活动。我们确定，Pα+/Krt5系（K5lin+）成纤维细胞对发育中的毛囊真皮干细胞生成真皮下乳头细胞和真皮下鞘细胞有显著贡献。在发育中的小鼠皮肤中，K5lin+成纤维细胞与毛囊发育同时出现，并在胚胎第16.5天形成早期真皮乳头的外缘细胞。K5lin+ 毛囊间充质细胞在老龄小鼠皮肤中也得以维持。这些结果为了解间充质和上皮之间的过渡在毛囊发育和维持中的作用和功能提供了启示。

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.